Moradi Sayyed Esmaeil, Shokrollahi Ardeshir, Shahdost-Fard Faezeh
Chemistry Department, Yasouj University, Yasouj, 75918-74831, Iran.
Department of Chemistry Education, Farhangian University, P.O. Box 14665-889, Tehran, Iran.
Heliyon. 2024 Nov 19;10(23):e40505. doi: 10.1016/j.heliyon.2024.e40505. eCollection 2024 Dec 15.
Detecting methadone (MET) is crucial due to its severe side effects.
Herein, a green nanocomposite based on reduced graphene (rGO) and β-cyclodextrin (β-CD) has been introduced to modify a glassy carbon electrode (GCE) for real-time measurement of MET. This eco-friendly sensing interface has synergistically benefited from both advantages of rGO and β-CD including excellent electron transfer tunneling and surface area enhancement to selectively trap MET based on its shape and size.
The developed sensor electrochemically detected MET at 0.8 V in buffer phosphate with a pH value of 7 under a wide linear concentration range (1 μM-830 μM), including a MET concentration level alarmed based on the consumer opioid tolerance according to the WHO's report. The limit of detection and analytical sensitivity values were calculated to be 333.33 nM and 0.0502 μA μM. The acceptable performance of the sensor to detect MET in various real human biofluids including serum, urine, and saliva samples, which is a bonus for the real-time and on-site measurement of MET, may open up a route for noninvasive routine tests in clinical samples. Moreover, the greenness profile of this strategy has been well evaluated by two common international metrics.
由于美沙酮（MET）具有严重的副作用，对其进行检测至关重要。
在此，一种基于还原氧化石墨烯（rGO）和β-环糊精（β-CD）的绿色纳米复合材料被用于修饰玻碳电极（GCE），以实现对美沙酮的实时检测。这种环保型传感界面协同受益于rGO和β-CD的优点，包括优异的电子转移隧道效应和表面积增强，能够基于美沙酮的形状和大小选择性地捕获它。
所开发的传感器在pH值为7的磷酸盐缓冲液中，于0.8 V电压下能够电化学检测美沙酮，其线性浓度范围较宽（1 μM - 830 μM），包括根据世界卫生组织报告中基于消费者阿片类药物耐受性而警示的美沙酮浓度水平。检测限和分析灵敏度值经计算分别为333.33 nM和0.0502 μA μM。该传感器在包括血清、尿液和唾液样本在内的各种真实人体生物流体中检测美沙酮的性能良好，这对于美沙酮的实时现场检测来说是一个优势，可能为临床样本的无创常规检测开辟一条途径。此外，该策略的绿色度已通过两个常见的国际指标得到了很好的评估。
