Andrzejuk Patrycja, Tokarska-Rodak Małgorzata, Dyrda Andżelika, Zarębska Marta
Innovation Research Centre, John Paul II University in Biała Podlaska, Biala Podlaska, Poland.
Faculty of Health Sciences, John Paul II University in Biała Podlaska, Biala Podlaska, Poland.
J Oral Microbiol. 2024 Dec 9;17(1):2437335. doi: 10.1080/20002297.2024.2437335. eCollection 2025.
spp., particularly , are commonly isolated fungi in the oral cavity. However, their prevalence in healthy participants and their genotype-phenotype relation remains elusive.
This study aimed to update the information on species colonizing the oral cavity of healthy population, identify the most common species, and characterize the intraspecific diversity to determine the genotype-phenotype relationship.
Oral swabs of healthy participants who declared an absence of oral infection were analyzed. Microbiological methods: chromogenic media, sugar assimilation tests, drug susceptibility, filamentation tests, temperature tolerance analysis, and assessment of biofilm formation ability. Genotyping methods: PCR amplification of the internal transcribed spacer (ITS) region with MspI restriction enzyme digestion and 25S rDNA region.
Of the 500 individuals tested, 130 harbored in 77%, in 12%, (previously ) in 8%, and (previously ) in 3%. The microbiological tests yielded conflicting results. Analysis of the 25S rDNA transposable intron region contributed to the identification of individual spp. and intraspecific identification of genotypes. Genotype A accounted for 70% ( = 100) of C. albicans isolates, whereas genotypes B, C, and D () accounted for 17%, 9%, and 4% of the isolates, respectively.
The results indicate a complex genotype-phenotype relationship in spp. and recommends combining microbiological and molecular methods for the efficient typing of spp.
某些菌种，尤其是白色念珠菌，是口腔中常见的分离真菌。然而，它们在健康受试者中的流行情况及其基因型-表型关系仍不明确。
本研究旨在更新关于健康人群口腔中定植的念珠菌属菌种的信息，确定最常见的菌种，并描述种内多样性以确定基因型-表型关系。
对宣称无口腔感染的健康受试者的口腔拭子进行分析。微生物学方法：显色培养基、糖同化试验、药敏试验、丝状化试验、温度耐受性分析以及生物膜形成能力评估。基因分型方法：用MspI限制性内切酶消化对内部转录间隔区（ITS）进行PCR扩增以及对25S rDNA区域进行分析。
在500名受试个体中，130人携带念珠菌，其中白色念珠菌占77%，热带念珠菌占12%，近平滑念珠菌（以前称为光滑念珠菌）占8%，季也蒙念珠菌（以前称为季也蒙假丝酵母）占3%。微生物学试验结果相互矛盾。对25S rDNA转座子内含子区域的分析有助于单个念珠菌属菌种的鉴定以及白色念珠菌基因型的种内鉴定。基因型A占白色念珠菌分离株的70%（n = 100），而基因型B、C和D（n = 48）分别占分离株的17%、9%和4%。
结果表明念珠菌属菌种存在复杂的基因型-表型关系，并建议结合微生物学和分子方法对念珠菌属菌种进行有效分型。
