Gunasekaran Gokul, Madhubala Manavalan Madhana, Nayanthara G S, Mahalaxmi Sekar
Department of Conservative Dentistry and Endodontics, SRM Dental College, Ramapuram, SRM Institute of Science and Technology, Chennai, India.
Aust Endod J. 2025 Apr;51(1):90-102. doi: 10.1111/aej.12910. Epub 2024 Dec 13.
This study aims to evaluate the effect of antimicrobial photodynamic therapy(aPDT) with polydopamine nanoparticle functionalised with Curcuma longa(nPD-Cur) against root canal biofilm. nPD-Cur was prepared and characterised using Scanning Electron Microscopy(SEM), dynamic light scattering(DLS), Fourier-transform infrared spectroscopy(FTIR) and Ultraviolet visual(UV/Vis) spectrophotometry. Root sections (10 mm length) were obtained from 53 single-rooted human premolars and chemo-mechanically prepared followed by inoculation with E. faecalis. All the specimens were randomly divided into five groups(n = 10) and irrigated (Group 1-Saline; Group 2-2.5% Sodium hypochlorite(NaOCl); Group 3-Cur; Group 4-nPD; and Group 5-nPD-Cur) followed by diode irradiation and analysed for reduction in colony-forming units(CFU)/mL, bacterial viability using Confocal Laser Scanning Microscopy(CLSM) and SEM for biofilm disruption. Results were analysed using one-way ANOVA followed by post hoc Tukey's test for pairwise comparison (p < 0.05). nPD-Cur revealed the characteristic absorption patterns. The antimicrobial potency was highest for NaOCl followed by nPD > Cur-nPD > Cur.
本研究旨在评估用姜黄功能化的聚多巴胺纳米颗粒（nPD-Cur）进行抗菌光动力疗法（aPDT）对根管生物膜的作用。制备了nPD-Cur，并使用扫描电子显微镜（SEM）、动态光散射（DLS）、傅里叶变换红外光谱（FTIR）和紫外可见（UV/Vis）分光光度法对其进行表征。从53颗单根人前磨牙获取牙根段（长度为10毫米），进行化学机械预备，然后接种粪肠球菌。所有标本随机分为五组（n = 10）并进行冲洗（第1组-生理盐水；第2组-2.5%次氯酸钠（NaOCl）；第3组-姜黄素（Cur）；第4组-nPD；第5组-nPD-Cur），随后进行二极管照射，并分析菌落形成单位（CFU）/毫升的减少情况，使用共聚焦激光扫描显微镜（CLSM）检测细菌活力，用SEM观察生物膜破坏情况。结果采用单因素方差分析，然后进行事后Tukey检验进行两两比较（p < 0.05）。nPD-Cur呈现出特征性吸收模式。抗菌效力最高的是NaOCl，其次是nPD＞姜黄素-nPD＞姜黄素。
