Hsieh Lan-Chi, Hsieh Shu-Ling, Ping Tsu-Ni, Huang Yi-Chun, Lin Ssu-Jung, Chi Hsing-Yu, Wu Chih-Chung
Department of Dietetics, Kaohsiung Municipal United Hospital, Kaohsiung, Taiwan, R.O.C.
Department of Seafood Science, National Kaohsiung University of Science and Technology, Kaohsiung, Taiwan, R.O.C.
Pharm Biol. 2025 Dec;63(1):1-13. doi: 10.1080/13880209.2024.2433994. Epub 2024 Dec 13.
L. (celery) is a dietary vegetable with anti-inflammatory properties. It has the potential to treat acute lung injury (ALI) caused by COVID-19 or other diseases.
To investigate the effects of water extract (AGWE) on ALI in human lung A-549 cells induced by lipopolysaccharide (LPS).
A-549 cells were treated with AGWE for 24 h and then stimulated with 10 μg/mL LPS for another 24 h. The effects of AGWE on cell viability, the inflammatory response, oxidative stress, and apoptosis and their regulatory factors, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), and NLR family pyrin domain containing 3 (NLRP3) inflammasome signaling activation were analyzed.
Treatment with 5-50 μg/mL AGWE reversed the decrease in cell viability caused by LPS ( < 0.05). AGWE can reduce interleukin (IL)-1β, IL-6, IL-18, and TNF-α levels; their EC values are 61.4, 65.7, 37.8, and 79.7 μg/mL, respectively. AGWE can reduce reactive oxygen species and thiobarbituric acid reactive substances in A-549 cells induced by LPS. AGWE also reduced the levels of apoptosis (EC50 of 74.8 μg/mL) and its regulators (Bid; Caspase-9, -8, and -3; Bax) and increased the levels of the mitochondrial membrane potential in A-549 cells induced by LPS. AGWE can also decrease the protein levels of NLRP3 and Caspase-1 and the activation of NF-κB signaling in A-549 cells induced by LPS.
These results show that 10 and 50 μg/mL AGWE can reduce the acute inflammation induced by LPS by reducing NF-κB and NLRP3 inflammasome signaling and mitochondria-dependent apoptosis pathways.
芹菜是一种具有抗炎特性的食用蔬菜。它具有治疗由新型冠状病毒肺炎或其他疾病引起的急性肺损伤（ALI）的潜力。
研究水提取物（AGWE）对脂多糖（LPS）诱导的人肺A-549细胞急性肺损伤的影响。
用AGWE处理A-549细胞24小时，然后用10μg/mL LPS再刺激24小时。分析AGWE对细胞活力、炎症反应、氧化应激、细胞凋亡及其调节因子、活化B细胞核因子κB（NF-κB）和含NLR家族pyrin结构域3（NLRP3）炎性小体信号激活的影响。
用5-50μg/mL AGWE处理可逆转LPS引起的细胞活力下降（P<0.05）。AGWE可降低白细胞介素（IL）-1β、IL-6、IL-18和肿瘤坏死因子-α水平；它们的半数有效浓度分别为61.4、65.7、37.8和79.7μg/mL。AGWE可降低LPS诱导的A-549细胞中的活性氧和硫代巴比妥酸反应性物质。AGWE还降低了LPS诱导的A-549细胞中的细胞凋亡水平（半数有效浓度为74.8μg/mL）及其调节因子（Bid；半胱天冬酶-9、-8和-3；Bax），并提高了线粒体膜电位水平。AGWE还可降低LPS诱导的A-549细胞中NLRP3和半胱天冬酶-1的蛋白水平以及NF-κB信号的激活。
这些结果表明，10和50μg/mL AGWE可通过降低NF-κB和NLRP3炎性小体信号以及线粒体依赖性细胞凋亡途径来减轻LPS诱导的急性炎症。
