基于过氧化物酶模拟活性的开-关策略用于检测六价铬的铜-姜黄素纳米酶

Cu-curcumin nanozyme for detection of Cr(VI) through an off-on strategy based on peroxidase mimicking activity.

作者信息

Chai Ziwei, Zhou Aifang, Huang Jingjing, Qu Lingbo, Ge Jia, Zhang Lin, Li Zhaohui

机构信息

College of Chemistry, Henan Joint International Research Laboratory of Green Construction of Functional Molecules and Their Bioanalytical Applications, Zhengzhou Key Laboratory of Functional Nanomaterial and Medical Theranostic, Food Laboratory of Zhongyuan, Zhengzhou University, Zhengzhou 450001, China.

Key Laboratory of Food Safety Quick Testing and Smart Supervision Technology for State Market Regulation, Henan Insitute of Food and Salt Industry Inspection Technology, Zhengzhou, 450003, China.

出版信息

Analyst. 2025 Jan 13;150(2):371-377. doi: 10.1039/d4an01358a.

DOI:10.1039/d4an01358a

PMID:39670858

Abstract

Hexavalent chromium ions (Cr(VI)) possess inherent toxicity and propensity for bioaccumulation in the environment. Herein, a Cu-curcumin nanozyme was synthesized a one-pot solvothermal method. Based on the high peroxidase-like activity of the Cu-curcumin nanozyme, a novel and economical colorimetric platform has been developed for Cr(VI) assay. In the presence of hydrogen peroxide (HO), the Cu-curcumin nanozyme can catalyze the transformation of colorless 3,3',5,5'-tetramethylbenzidine (TMB) into the blue product oxTMB. 8-Hydroxyquinoline (8-HQ) can inhibit the catalysis of the Cu-curcumin/TMB/HO system, causing the blue color to fade. With the addition of Cr(VI), the peroxidase-like activity of the Cu-curcumin nanozyme is increased significantly, leading to the restoration of the Cu-curcumin/TMB/HO system color. After optimization of the experimental parameters, a novel colorimetric sensor for Cr(VI) assay has been successfully developed with a low limit of detection (LOD) of 31.5 nM, affirming its potential for accurate Cr(VI) quantification in environmental specimens. In addition, paper strips integrated with a smartphone platform enhance the versatility and portability of the Cr(VI) assay, achieving an LOD of 0.1 μM.

摘要

六价铬离子（Cr(VI)）具有内在毒性且易于在环境中生物累积。在此，采用一锅法溶剂热法合成了一种铜 - 姜黄素纳米酶。基于铜 - 姜黄素纳米酶的高类过氧化物酶活性，开发了一种用于检测Cr(VI)的新型经济比色平台。在过氧化氢（H₂O₂）存在下，铜 - 姜黄素纳米酶可催化无色的3,3',5,5'-四甲基联苯胺（TMB）转化为蓝色产物氧化型TMB（oxTMB）。8 - 羟基喹啉（8 - HQ）可抑制铜 - 姜黄素/TMB/H₂O₂体系的催化作用，使蓝色褪色。加入Cr(VI)后，铜 - 姜黄素纳米酶的类过氧化物酶活性显著增强，导致铜 - 姜黄素/TMB/H₂O₂体系颜色恢复。经实验参数优化后，成功开发了一种用于检测Cr(VI)的新型比色传感器，其检测限低至31.5 nM，证实了其在环境样品中准确定量Cr(VI)的潜力。此外，与智能手机平台集成的试纸条提高了Cr(VI)检测的通用性和便携性，检测限达到0.1 μM。