Xu Jiao, Yu Xue-Jie
State Key Laboratory of Virology, School of Public Health, Wuhan University, Wuhan City, Hubei Province, China.
Methods Mol Biol. 2025;2893:109-117. doi: 10.1007/978-1-0716-4338-9_9.
Many members in the class Bunyaviricetes are tick-borne viruses. Experimental viral infection of ticks is greatly needed to better understand the tick-virus interactions. To date, multiple laboratory techniques for the viral infection of ticks have been documented. Here we describe two methods of infecting Haemaphysalis longicornis ticks with SFTSV: infecting ticks through microinjection and direct feeding on infected animal hosts. Both methods have been shown to be effective in infecting ticks.
布尼亚病毒目（Bunyaviricetes）中的许多病毒成员是蜱传病毒。为了更好地了解蜱与病毒之间的相互作用，非常需要对蜱进行实验性病毒感染。迄今为止，已经记录了多种用于蜱病毒感染的实验室技术。在此，我们描述了两种用发热伴血小板减少综合征病毒（SFTSV）感染长角血蜱（Haemaphysalis longicornis）的方法：通过显微注射感染蜱以及让蜱直接取食受感染的动物宿主。这两种方法都已证明在感染蜱方面是有效的。
