Rohini C, Kavya T A, Kumar A Niranjana, Kumar J Kotesh, Satya Srinivas K V N, Venkatesh B, Ravi Kumar P
Pharmaceutical Analysis, G.Pulla Reddy College of Pharmacy, Hyderabad, India.
Phytochemistry Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Research Centre, Boduppal, Hyderabad, India.
Nat Prod Res. 2024 Dec 13:1-7. doi: 10.1080/14786419.2024.2440521.
Nees (Acanthaceae), an ayurvedic shrub, is used widely in respiratory disorders. The pharmacologically most studied phytoconstituent in is vasicine, a bitter quinazoline alkaloid present in leaves. A robust method was developed for the simultaneous quantification and analysis of two major alkaloids i.e. vasicine and vasicinone by RP-HPLC. This paper describes the distribution of these markers in different parts of the plant along with the best extraction method for their recovery. The highest abundance of vasicine was observed in the leaves (0.7332%), than in the stems and roots, whereas vasicinone was present only in the leaves (0.0436%). Methanol was identified as the most effective solvent for the extraction of vasicine in successive extraction (the herb was extracted sequentially with increasing solvent polarity) whereas 30% aq. Methanol is recommended for extraction of vasicinone in individual solvent extraction (the fresh herb was extracted separately with different solvents) method.
假杜鹃（爵床科）是一种阿育吠陀灌木，广泛用于治疗呼吸系统疾病。其中在药理学上研究最多的植物成分是鸭嘴花碱，一种存在于叶片中的苦味喹唑啉生物碱。开发了一种通过反相高效液相色谱法同时定量和分析两种主要生物碱即鸭嘴花碱和去甲鸭嘴花碱的可靠方法。本文描述了这些标志物在植物不同部位的分布情况以及用于它们回收的最佳提取方法。鸭嘴花碱的含量在叶片中最高（0.7332%），高于茎和根，而去甲鸭嘴花碱仅存在于叶片中（0.0436%）。在连续提取中（随着溶剂极性增加依次提取草药），甲醇被确定为提取鸭嘴花碱最有效的溶剂，而在单独溶剂提取法（用不同溶剂分别提取新鲜草药）中，推荐用30%的甲醇水溶液提取去甲鸭嘴花碱。
