Zhou Bosen, Yang Yi, Yu Liansheng, Song Gang, Ge Jingping, Du Renpeng
Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region & Key Laboratory of Microbiology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin 150080, China.
Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region & Key Laboratory of Microbiology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin 150080, China.
Int J Biol Macromol. 2025 Feb;289:138685. doi: 10.1016/j.ijbiomac.2024.138685. Epub 2024 Dec 11.
Bacterial cellulose (BC) is a natural, renewable polymer material with an ultrafine nanonetwork structure. However, BC has limited applications in food packaging and medical materials because of its lack of antibacterial properties. To expand the applications of BC, a new BC composite membrane was synthesized via an ex situ method. The BC membrane was first immersed in 100 mL of deionized water containing 3 mg of AgNO₃ for 24 h to incorporate silver nanoparticles (AgNPs). The BC-Ag composite was immersed in a 2 wt% montmorillonite (MMT) solution for 24 h to prepare the BC-Ag-MMT composite membrane. The structure and antibacterial properties of the composite were then examined. Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM) and transmission electron microscopy (TEM) confirmed the successful synthesis of the BC-Ag-MMT composite membrane. The results revealed that the addition of AgNPs and MMT resulted in a significant decrease in porosity (13.23 ± 1.12 %), water vapor transmission rate (735.12 ± 12.55 g/(m·day)), and oxygen permeability (2.28 ± 0.29 g/(s·mPa)) while increasing the membrane thickness (0.89 ± 0.08 mm). The incorporation of MMT into BC notably improved the thermal stability of the membrane and further influenced its porosity. The antibacterial activity was evaluated via the inhibition zone method. The BC-Ag-MMT composite membrane exhibited antibacterial activity against Salmonella paratyphi A, Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Salmonella enterica. These findings demonstrated that the BC-Ag-MMT composite membrane possesses exceptional physical and chemical properties, mechanical strength, and antibacterial efficacy. The composite membrane holds significant potential for applications in food packaging.
细菌纤维素（BC）是一种具有超细纳米网络结构的天然可再生聚合物材料。然而，由于缺乏抗菌性能，BC在食品包装和医疗材料中的应用有限。为了拓展BC的应用，通过非原位方法合成了一种新型BC复合膜。首先将BC膜浸入含有3mg硝酸银的100mL去离子水中24小时，以掺入银纳米颗粒（AgNPs）。将BC-Ag复合材料浸入2wt%的蒙脱石（MMT）溶液中24小时，制备BC-Ag-MMT复合膜。然后对该复合材料的结构和抗菌性能进行了研究。傅里叶变换红外（FT-IR）光谱、X射线衍射（XRD）、X射线光电子能谱（XPS）、扫描电子显微镜（SEM）和透射电子显微镜（TEM）证实了BC-Ag-MMT复合膜的成功合成。结果表明，添加AgNPs和MMT导致孔隙率（13.23±1.12%）、水蒸气透过率（735.12±12.55g/(m·天)）和氧气透过率（2.28±0.29g/(s·mPa)）显著降低，同时增加了膜厚度（0.89±0.08mm）。将MMT掺入BC中显著提高了膜的热稳定性，并进一步影响了其孔隙率。通过抑菌圈法评估抗菌活性。BC-Ag-MMT复合膜对甲型副伤寒沙门氏菌、大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌和肠炎沙门氏菌具有抗菌活性。这些发现表明，BC-Ag-MMT复合膜具有优异的物理和化学性能、机械强度和抗菌效果。该复合膜在食品包装应用中具有巨大潜力。
