Schmidt G H, Wilkinson M M, Ponder B A
Cell. 1985 Feb;40(2):425-9. doi: 10.1016/0092-8674(85)90156-4.
The cell migration pathway in the intestinal epithelium of DDK in equilibrium C57BL/6JLac mouse chimeras is demonstrated using Dolichos biflorus agglutinin-peroxidase as strain-specific marker. Cell sheets of one genotype extend in relatively straight lines from crypt to villus apex. Narrow sheets are mostly interrupted in the distal two-thirds of duodenal but not ileal villi, suggesting that in the duodenum cell loss occurs below the apical extrusion zone. These differences between duodenum and ileum correspond to differences in villus shape. The pattern of cell migration in Peyer's patch epithelium is consistent with that of the duodenum. In chimeric colon, sharply demarcated territories of crypts with a narrow cuff of surface epithelium represent the counterpart of the villus/crypt unit of the small intestine.
利用双花扁豆凝集素-过氧化物酶作为品系特异性标记，证明了处于平衡状态的C57BL/6JLac小鼠嵌合体中DDK肠道上皮细胞的迁移途径。一种基因型的细胞片从隐窝到绒毛顶端呈相对直线延伸。狭窄的细胞片在十二指肠远端三分之二的绒毛中大多中断，但在回肠绒毛中则不然，这表明在十二指肠中，细胞丢失发生在顶端挤出区下方。十二指肠和回肠之间的这些差异与绒毛形状的差异相对应。派尔集合淋巴结上皮中的细胞迁移模式与十二指肠一致。在嵌合结肠中，表面上皮狭窄袖口的隐窝界限分明的区域代表小肠绒毛/隐窝单元的对应物。
