Enhanced oxygen availability and preserved aggregative function in platelet concentrates stored at reduced platelet concentration.

BACKGROUND

Storage of platelets as platelet concentrates for transfusion is limited to 7 days in the United Kingdom due to deleterious effects on platelet quality and function that occur over time. Oxygen (O) availability and sufficient gaseous exchange are known to be essential in maintaining the viability and function of platelets stored for transfusion. Despite this, there is a paucity of studies undertaking direct measures of O and optimization of conditions throughout storage. We address this and modulate the storage conditions to improve platelet quality and function.

STUDY DESIGN AND METHODS

Electron paramagnetic resonance oximetry was implemented to directly measure the [O] experienced by stored platelet concentrates and the O consumption rate under standard blood banking conditions. From these direct measures the mathematical modeling was then applied to predict the main parameters contributing to effective O distribution throughout the unit.

RESULTS

This study demonstrates reducing the storage [O] to reflect near physiological levels significantly alters O distribution within the unit and negatively impacts platelet functionality and quality, and therefore is not a viable storage option.

DISCUSSION

We show the reduction of platelet concentration within a unit improves O availability and pH, promotes a more uniform distribution of O throughout prolonged storage, and maintains platelet agonist-induced aggregation comparable to 100% platelet concentration. This may be a viable option and could potentially lead to reduced donor demand.