Nash Jamie, Pym Dean, Davies A, Saunders Christine, George Chloe, Williams J O, Grinberg O Y, James Philip E
Center for Cardiovascular Health and Ageing, Cardiff Metropolitan University, Cardiff, UK.
Component Development and Research Laboratory, Welsh Blood Service NHS, Wales, UK.
Transfusion. 2025 Mar;65(3):575-587. doi: 10.1111/trf.18101. Epub 2024 Dec 14.
Storage of platelets as platelet concentrates for transfusion is limited to 7 days in the United Kingdom due to deleterious effects on platelet quality and function that occur over time. Oxygen (O) availability and sufficient gaseous exchange are known to be essential in maintaining the viability and function of platelets stored for transfusion. Despite this, there is a paucity of studies undertaking direct measures of O and optimization of conditions throughout storage. We address this and modulate the storage conditions to improve platelet quality and function.
Electron paramagnetic resonance oximetry was implemented to directly measure the [O] experienced by stored platelet concentrates and the O consumption rate under standard blood banking conditions. From these direct measures the mathematical modeling was then applied to predict the main parameters contributing to effective O distribution throughout the unit.
This study demonstrates reducing the storage [O] to reflect near physiological levels significantly alters O distribution within the unit and negatively impacts platelet functionality and quality, and therefore is not a viable storage option.
We show the reduction of platelet concentration within a unit improves O availability and pH, promotes a more uniform distribution of O throughout prolonged storage, and maintains platelet agonist-induced aggregation comparable to 100% platelet concentration. This may be a viable option and could potentially lead to reduced donor demand.
在英国，由于随着时间推移血小板质量和功能会受到有害影响，血小板浓缩物作为输血用血小板的储存期限限制为7天。已知氧气（O）的可利用性和充分的气体交换对于维持储存用于输血的血小板的活力和功能至关重要。尽管如此，很少有研究对整个储存过程中的氧气进行直接测量并优化条件。我们针对此问题并调节储存条件以改善血小板质量和功能。
采用电子顺磁共振血氧测定法直接测量储存的血小板浓缩物所经历的[O]以及标准血库条件下的氧气消耗率。然后根据这些直接测量结果应用数学模型来预测有助于单位内有效氧气分布的主要参数。
本研究表明，将储存的[O]降低至接近生理水平会显著改变单位内的氧气分布，并对血小板功能和质量产生负面影响，因此不是一种可行的储存选择。
我们表明，降低单位内的血小板浓度可提高氧气可利用性和pH值，在延长的储存过程中促进氧气更均匀的分布，并维持血小板激动剂诱导的聚集，与100%血小板浓度相当。这可能是一种可行的选择，并可能潜在地减少对供体的需求。
