Lavilla-Puerta Mikel, Giuntoli Beatrice
Plant Molecular Biology Section, Department of Biology, University of Oxford, OX1 3RB Oxford, UK.
Biology Department, University of Pisa, 56126 Pisa, Italy.
Plant Physiol. 2024 Dec 23;197(1). doi: 10.1093/plphys/kiae623.
Over the past years, plant hypoxia research has produced a considerable number of new resources to monitor low oxygen responses in model species, mainly Arabidopsis thaliana. Climate change urges the development of effective genetic strategies aimed at improving plant resilience during flooding events. This need pushes forward the search for optimized tools that can reveal the actual oxygen available to plant cells, in different organs or under various conditions, and elucidate the mechanisms underlying plant hypoxic responses, complementing the existing transcriptomics, proteomics, and metabolic analysis methods. Oxygen-responsive reporters, dyes, and nanoprobes are under continuous development, as well as novel synthetic strategies that make precision control of plant hypoxic responses realistic. In this review, we summarize the recent progress made in the definition of tools for oxygen response monitoring in plants, either adapted from bacterial and animal research or peculiar to plants. Moreover, we highlight how adoption of a synthetic biology perspective has enabled the design of novel genetic circuits for the control of oxygen-dependent responses in plants. Finally, we discuss the current limitations and challenges toward the implementation of synbio solutions in the plant low-oxygen biology field.
在过去几年中，植物缺氧研究产生了大量用于监测模式植物（主要是拟南芥）低氧反应的新资源。气候变化促使人们开发有效的遗传策略，以提高植物在洪水事件中的恢复力。这种需求推动了对优化工具的探索，这些工具能够揭示植物细胞在不同器官或各种条件下实际可利用的氧气，并阐明植物缺氧反应的潜在机制，以补充现有的转录组学、蛋白质组学和代谢分析方法。氧反应报告基因、染料和纳米探针正在不断发展，同时新的合成策略也使精确控制植物缺氧反应成为现实。在这篇综述中，我们总结了在植物氧反应监测工具定义方面取得的最新进展，这些工具要么改编自细菌和动物研究，要么是植物特有的。此外，我们强调了采用合成生物学观点如何能够设计新的遗传电路来控制植物中的氧依赖性反应。最后，我们讨论了在植物低氧生物学领域实施合成生物学解决方案目前的局限性和挑战。
