Ramgopal N Ch, Mukherjee Poulami, Kamilya Tapas, Basavegowda Nagaraj, Mahanthappa Mallappa, Aljarba Nada H, Alqahtani Reem A, Alkahtani Saad, Park Jinhyoung, Vishwanath R S
MLR Institute of Technology, Hyderabad, Telangana 500043, India.
Japan Advanced Institute of Science and Technology, Japan.
Int J Biol Macromol. 2025 Feb;288:138766. doi: 10.1016/j.ijbiomac.2024.138766. Epub 2024 Dec 12.
Nanozymes represent a compelling alternative to natural enzymes due to their exceptional stability and cost-efficiency in diagnostic and therapeutic applications. In this work, a nanozyme etched CoFe Prussian blue analog (etched PBA) was designed and fabricated by a simple approach involving first synthesizing CoFe PBA and then chemical etching. The fabricated etched PBA acts as a peroxidase mimic catalyst. Compared to the naturally occurring enzyme (horseradish peroxidase), the etched PBA exhibited improved peroxidase-like catalytic efficiency for oxidizing 3,3',5,5'-tetramethylbnmenzidine (TMB). Particularly, the exceptional peroxidase-like activity of the etched PBA is attributed to modifications in its electronic and structural properties, which improved affinity for hydrogen peroxide (HO) and TMB. Hence, these findings highlight etched PBA as a promising peroxidase mimic, enabling a colorimetric assay for real-time HO and dopamine (DA) detection. HO was detected via PBA-catalyzed TMB oxidation (bluish-green color), while DA monitoring occurred through ox-TMB reduction. The detection thresholds for HO and DA were determined to be 0.083 μM and 0.05 μM, respectively, with broad linear ranges of 0.1 μM - 4.0 mM for HO and 0.1-240 μM for DA. In addition, the etched PBA was validated for real sample analysis, showing potential for enzyme catalysis, biosensing, and colorimetric analysis of pharmaceuticals and biomolecules.
由于纳米酶在诊断和治疗应用中具有出色的稳定性和成本效益，它们成为天然酶极具吸引力的替代品。在这项工作中，通过一种简单的方法设计并制备了一种纳米酶蚀刻钴铁普鲁士蓝类似物（蚀刻PBA），该方法首先合成钴铁PBA，然后进行化学蚀刻。制备的蚀刻PBA用作过氧化物酶模拟催化剂。与天然存在的酶（辣根过氧化物酶）相比，蚀刻PBA在氧化3,3',5,5'-四甲基联苯胺（TMB）方面表现出更高的过氧化物酶样催化效率。特别地，蚀刻PBA异常的过氧化物酶样活性归因于其电子和结构性质的改变，这提高了对过氧化氢（HO）和TMB的亲和力。因此，这些发现突出了蚀刻PBA作为一种有前景的过氧化物酶模拟物，能够用于实时HO和多巴胺（DA）检测的比色测定。通过PBA催化的TMB氧化（蓝绿色）检测HO，而通过氧化TMB的还原监测DA。HO和DA的检测限分别确定为0.083 μM和0.05 μM，HO的线性范围为0.1 μM - 4.0 mM，DA的线性范围为0.1 - 240 μM。此外，蚀刻PBA经过实际样品分析验证，显示出在酶催化、生物传感以及药物和生物分子比色分析方面的潜力。
