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磷酸二羟丙酮酰基转移酶活性在雄性和雌性小鼠肾过氧化物酶体中的主要定位。

Predominant localization of dihydroxyacetone-phosphate acyltransferase activity in renal peroxisomes of male and female mice.

作者信息

Mackness M I, Connock M J

出版信息

Comp Biochem Physiol B. 1985;80(1):161-4. doi: 10.1016/0305-0491(85)90439-0.

Abstract

The subcellular localization of DHAPAT activity in male and female albino mouse kidneys was investigated by density gradient centrifugation. DHAPAT has a predominantly peroxisomal distribution in both male and female kidneys; however some activity is also distributed in a less dense region of the gradient, predominantly containing microsomes. Peroxisomal fractions also contain some lactate dehydrogenase activity and approximately 9% of the cellular NAD-dependent alpha-glycerophosphate dehydrogenase activity.

摘要

通过密度梯度离心法研究了二羟丙酮磷酸酰基转移酶(DHAPAT)活性在雄性和雌性白化小鼠肾脏中的亚细胞定位。DHAPAT在雄性和雌性肾脏中主要分布于过氧化物酶体;然而,一些活性也分布在梯度密度较低的区域,该区域主要含有微粒体。过氧化物酶体组分还含有一些乳酸脱氢酶活性以及约9%的细胞NAD依赖性α-甘油磷酸脱氢酶活性。

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