Eco-friendly antibacterial electrospinning nanofibrous film containing nano-silver green-synthesized by natural glycoprotein for infected wound healing.

Infected wounds caused by bacteria commonly cause delayed wound healing and even serious tissue damage, evolving into one of the most problematic clinical issues. Traditional therapies and wound dressings cannot achieve either the ideal antibiotic activity or the accelerated wound healing outcomes. Based on our previous finding that glycoproteins (PAGP) derived from medicinal animal insect Periplaneta americana L. have a significant promoting effect on wound healing, herein we primarily prepared the silver nanoparticles (Ag NPs) by the facile green-synthesis mediated by PAGP, and then the eco-friendly PAGP nanofibrous film inlaid with Ag NPs (Ag NPs@PP film) was fabricated by electrospinning. The obtained nanofibrous dressing had good mechanical properties, surface wettability, biocompatibility, and antibacterial properties. Compared with nanofiber film containing only Ag NPs or PAGP, the Ag NPs@PP film had stronger abilities to promote the M2 polarization of macrophages and higher wound-healing efficacy on fecal bacteria-infected wounds. To sum up, this nanofibrous film held great promise to improve the healing of infected wounds.