Li Xuebo, Pang Lan, Duan Jia, Huang Na, Chen Xiangyu, Huang Wei, Liu Yang, Fu Chaomei, Zhang Chen, Tu He, Zeng Chenjuan, Liu Xinjun, Zhang Jinming
State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China; Sichuan Key Laboratory for Medicinal American Cockroach, Chengdu 610000, China.
J Colloid Interface Sci. 2025 Apr;683(Pt 1):256-268. doi: 10.1016/j.jcis.2024.12.019. Epub 2024 Dec 6.
Infected wounds caused by bacteria commonly cause delayed wound healing and even serious tissue damage, evolving into one of the most problematic clinical issues. Traditional therapies and wound dressings cannot achieve either the ideal antibiotic activity or the accelerated wound healing outcomes. Based on our previous finding that glycoproteins (PAGP) derived from medicinal animal insect Periplaneta americana L. have a significant promoting effect on wound healing, herein we primarily prepared the silver nanoparticles (Ag NPs) by the facile green-synthesis mediated by PAGP, and then the eco-friendly PAGP nanofibrous film inlaid with Ag NPs (Ag NPs@PP film) was fabricated by electrospinning. The obtained nanofibrous dressing had good mechanical properties, surface wettability, biocompatibility, and antibacterial properties. Compared with nanofiber film containing only Ag NPs or PAGP, the Ag NPs@PP film had stronger abilities to promote the M2 polarization of macrophages and higher wound-healing efficacy on fecal bacteria-infected wounds. To sum up, this nanofibrous film held great promise to improve the healing of infected wounds.
由细菌引起的感染性伤口通常会导致伤口愈合延迟，甚至造成严重的组织损伤，进而演变成最具问题的临床问题之一。传统疗法和伤口敷料既无法实现理想的抗生素活性，也无法达成加速伤口愈合的效果。基于我们之前的发现，即源自药用动物昆虫美洲大蠊的糖蛋白（PAGP）对伤口愈合具有显著的促进作用，在此我们首先通过PAGP介导的简便绿色合成法制备了银纳米颗粒（Ag NPs），然后通过静电纺丝制备了镶嵌有Ag NPs的环保型PAGP纳米纤维膜（Ag NPs@PP膜）。所获得的纳米纤维敷料具有良好的机械性能、表面润湿性、生物相容性和抗菌性能。与仅含有Ag NPs或PAGP的纳米纤维膜相比，Ag NPs@PP膜具有更强的促进巨噬细胞M2极化的能力，并且对粪便细菌感染的伤口具有更高的伤口愈合效果。综上所述，这种纳米纤维膜在改善感染性伤口愈合方面具有巨大潜力。
