Kheradvar Kolour Alireza, Ghoraishizadeh Saman, Zaman Mohammad Sadegh, Alemzade Amirata, Banavand Mozhgan, Esmaeili Javad, Shahrousvand Mohsen
Caspian Faculty of Engineering, College of Engineering, University of Tehran, Rezvanshar, Guilan 43861-91836, Iran.
Tissue Engineering Department, TISSUEHUB Co., Tehran 19568-54977, Iran.
ACS Appl Bio Mater. 2024 Dec 16;7(12):8642-8655. doi: 10.1021/acsabm.4c01449. Epub 2024 Nov 12.
A promising approach for wound treatment is using multilayer wound dressings that offer multifunctional properties. In this study, a bilayered electrospun/hydrogel gelatin-based scaffold integrated with honey and curcumin was developed to treat wounds under an in vivo study. The first layer consisted of an enzymatic cross-linked gelatin hydrogel containing honey and curcumin, which gelatin/PCL nanofibers reinforced. The physicochemical, mechanical, and biological properties of both layers were evaluated. Then, the bilayered wound dressing was compared to a commercial wound dressing in an in vivo study. The results showed that this strategy provided the wound dressing with a strength of 40 MPa, 70% elongation, 800% swelling rate, and 8 g/h/m water vapor permeability. Furthermore, MTT and histopathological staining demonstrated that the bilayered wound dressing promoted wound closure accelerated collagen production and tissue granulation, and promoted immune system response and re-epithelialization compared to other groups. The presence of a nanofibrous layer on the surface of the wound dressing facilitated its use, and the inclusion of honey and gelatin in the hydrogel layer prevented adhesion to the wound tissue and allowed for easy replacement without damaging the wound bed. Overall, the bilayered dressing with multifunctional properties holds great potential for developing wound dressings.
一种很有前景的伤口治疗方法是使用具有多功能特性的多层伤口敷料。在本研究中，开发了一种集成了蜂蜜和姜黄素的双层电纺/水凝胶明胶基支架，用于在体内研究中治疗伤口。第一层由含有蜂蜜和姜黄素的酶交联明胶水凝胶组成，明胶/聚己内酯纳米纤维对其进行了增强。对两层的物理化学、机械和生物学特性进行了评估。然后，在体内研究中将双层伤口敷料与市售伤口敷料进行了比较。结果表明，该策略赋予伤口敷料40兆帕的强度、70%的伸长率、800%的溶胀率和8克/小时/平方米的水蒸气透过率。此外，MTT和组织病理学染色表明，与其他组相比，双层伤口敷料促进了伤口闭合，加速了胶原蛋白生成和组织肉芽形成，并促进了免疫系统反应和再上皮化。伤口敷料表面存在纳米纤维层便于其使用，水凝胶层中包含蜂蜜和明胶可防止与伤口组织粘连，并允许在不损伤伤口床的情况下轻松更换。总体而言，具有多功能特性的双层敷料在伤口敷料开发方面具有巨大潜力。
