Almeida Edelbert A, Mehndiratta Mohit, Madhu Sri V, Kar Rajarshi
Department of Biochemistry, University College of Medical Sciences and GTB Hospital, University of Delhi, Delhi, India.
Department of Endocrinology, University College of Medical Sciences and GTB Hospital, University of Delhi, Delhi, India.
Indian J Endocrinol Metab. 2024 Sep-Oct;28(5):517-521. doi: 10.4103/ijem.ijem_474_23. Epub 2024 Sep 4.
Type 2 diabetes mellitus (T2DM) is closely associated with the obesity; however, certain proportion of T2DM patients is non-obese or lean (BMI < 18.5 kg/m). Obesity has long been associated with oxidative stress; however, there are no studies available documenting levels of oxidative stress in the lean patients of T2DM. Therefore, this study was done to compare the levels of makers of oxidative stress (TL, mtDNA, TAS) and their regulators (mRNA expression of and ) in lean and obese patients of T2DM.
60 newly diagnosed patients (treatment naïve) of T2DM were recruited and divided into lean (BMI < 18.5 kg/m) and obese (BMI > 25 kg/m) groups. Relative telomere length (T/S) and mtDNA content were estimated via real-time PCR. Serum total antioxidant status (TAS) was measured using a commercially available kit. mRNA expression of and was measured by real-time PCR.
Mean T/S and mtDNA content were lower in the obese group compared to the lean group ( = 0.16 and = 0.06, respectively). Mean serum TAS levels were higher in obese group compared to the lean group ( = 0.001). mRNA expression of and was increased in obese group compared to the lean group. mRNA expression of was similar in both the groups.
Obese patients of T2DM are exposed to a greater degree of OS compared to the lean patients of T2DM.
2型糖尿病（T2DM）与肥胖密切相关；然而，一定比例的T2DM患者并非肥胖或体型偏瘦（体重指数<18.5kg/m²）。肥胖长期以来一直与氧化应激相关；然而，尚无研究记录T2DM瘦患者的氧化应激水平。因此，本研究旨在比较T2DM瘦患者和肥胖患者氧化应激标志物（端粒长度、线粒体DNA、总抗氧化能力）及其调节因子（[具体基因1]和[具体基因2]的mRNA表达）的水平。
招募60例新诊断的T2DM患者（未接受过治疗），并分为瘦组（体重指数<18.5kg/m²）和肥胖组（体重指数>25kg/m²）。通过实时聚合酶链反应估计相对端粒长度（T/S）和线粒体DNA含量。使用市售试剂盒测量血清总抗氧化状态（TAS）。通过实时聚合酶链反应测量[具体基因1]和[具体基因2]的mRNA表达。
与瘦组相比，肥胖组的平均T/S和线粒体DNA含量较低（分别为P = 0.16和P = 0.06）。与瘦组相比，肥胖组的平均血清TAS水平较高（P = 0.001）。与瘦组相比，肥胖组中[具体基因1]和[具体基因2]的mRNA表达增加。两组中[具体基因3]的mRNA表达相似。
与T2DM瘦患者相比，T2DM肥胖患者暴露于更高程度的氧化应激。
