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通过实验和计算方法增强的代谢纳米显微镜技术。

Metabolic nanoscopy enhanced by experimental and computational approaches.

作者信息

Jang Hongje, Wu Shuang, Li Yajuan, Li Zhi, Shi Lingyan

机构信息

Shu Chien - Gene Lay Department of Bioengineering, University of California San Diego, La Jolla, CA USA.

出版信息

Npj Imaging. 2024;2(1):55. doi: 10.1038/s44303-024-00062-y. Epub 2024 Dec 12.

DOI:10.1038/s44303-024-00062-y
PMID:39677560
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11638068/
Abstract

The advances in microscopy techniques have led to new findings in biology. The recent advances in super-resolution microscopy technologies revealed precise molecular distribution. The techniques to visualize the distributions of multiple molecules in biological samples from experimental techniques to computational approaches are reviewed. By summarizing the techniques, the future direction of collaborative research of the techniques is highlighted to show the nanoscopic chemical details of biological samples.

摘要

显微镜技术的进步带来了生物学领域的新发现。超分辨率显微镜技术的最新进展揭示了精确的分子分布。本文综述了从实验技术到计算方法的用于可视化生物样品中多种分子分布的技术。通过总结这些技术,突出了该技术协同研究的未来方向,以展示生物样品的纳米级化学细节。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f1a/12118723/d315e62f9317/44303_2024_62_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f1a/12118723/90171597de30/44303_2024_62_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f1a/12118723/69c6a0b87e5c/44303_2024_62_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f1a/12118723/c7a77edefcd0/44303_2024_62_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f1a/12118723/d315e62f9317/44303_2024_62_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f1a/12118723/90171597de30/44303_2024_62_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f1a/12118723/69c6a0b87e5c/44303_2024_62_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f1a/12118723/c7a77edefcd0/44303_2024_62_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f1a/12118723/d315e62f9317/44303_2024_62_Fig4_HTML.jpg

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本文引用的文献

1
Multi-molecular hyperspectral PRM-SRS microscopy.多分子高光谱 PRM-SRS 显微镜。
Nat Commun. 2024 Feb 21;15(1):1599. doi: 10.1038/s41467-024-45576-6.
2
Super-Resolution Stimulated Raman Scattering Microscopy with Graphical User Interface-Supported A-PoD.带图形用户界面支持的 A-PoD 的超分辨率受激拉曼散射显微镜
Curr Protoc. 2024 Jan;4(1):e970. doi: 10.1002/cpz1.970.
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Multimodal imaging of metabolic activities for distinguishing subtypes of breast cancer.用于区分乳腺癌亚型的代谢活动多模态成像
Biomed Opt Express. 2023 Oct 13;14(11):5764-5780. doi: 10.1364/BOE.500252. eCollection 2023 Nov 1.
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Current capabilities and future perspectives of FCS: super-resolution microscopy, machine learning, and in vivo applications.当前 FCS 的能力和未来展望:超分辨率显微镜、机器学习和活体应用。
Commun Biol. 2023 Jul 7;6(1):699. doi: 10.1038/s42003-023-05069-6.
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Bioorthogonal Stimulated Raman Scattering Imaging Uncovers Lipid Metabolic Dynamics in Brain During Aging.生物正交刺激拉曼散射成像揭示衰老过程中大脑的脂质代谢动态。
GEN Biotechnol. 2023 Jun 1;2(3):247-261. doi: 10.1089/genbio.2023.0017. Epub 2023 Jun 19.
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Measurement of chemical penetration in skin using Stimulated Raman scattering microscopy and multivariate curve resolution - alternating least squares.使用受激拉曼散射显微镜和多元曲线分辨-交替最小二乘法测量皮肤中的化学渗透。
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Super-resolution SRS microscopy with A-PoD.基于 A-PoD 的超分辨率 SRS 显微镜技术
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A Highly Sensitive Fluorescent Akt Biosensor Reveals Lysosome-Selective Regulation of Lipid Second Messengers and Kinase Activity.一种高灵敏度荧光Akt生物传感器揭示了溶酶体对脂质第二信使和激酶活性的选择性调控。
ACS Cent Sci. 2021 Dec 22;7(12):2009-2020. doi: 10.1021/acscentsci.1c00919. Epub 2021 Dec 3.
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Sparse deconvolution improves the resolution of live-cell super-resolution fluorescence microscopy.稀疏反卷积提高了活细胞超分辨率荧光显微镜的分辨率。
Nat Biotechnol. 2022 Apr;40(4):606-617. doi: 10.1038/s41587-021-01092-2. Epub 2021 Nov 15.
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