Li Boran, Gao Wenyan, Pan Yuanyuan, Yao Yongpeng, Liu Gang
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China.
Front Bioeng Biotechnol. 2024 Nov 29;12:1507680. doi: 10.3389/fbioe.2024.1507680. eCollection 2024.
1,3-Propanediol (1,3-PDO) is one of the important organic chemical materials and is widely used in polyester synthesis, and it also shows great potential in medicine, cosmetics, resins, and biodegradable plastics. So far, 1,3-PDO mainly comes from chemical synthesis. However, the by-products and the side effects during chemical synthesis of 1,3-PDO bring about serious damage to the environment. In recent years, the biosynthetic pathway of 1,3-PDO has been elucidated in microorganisms. Under the action of glycerol dehydratase (GDHt) and propanediol oxidoreductase (PDOR), glycerol can be catalyzed to form 1,3-PDO through the reduction pathway. Compared to the chemical synthesis, the biosynthesis of 1,3-PDO is environmentally friendly but would face the problem of low production. To improve the yield, the native 1,3-PDO producing strains have been modified by genetic engineering, and the biosynthetic pathway has been reconstructed in the model microorganism, . In this review, we summarize the research progress of the 1,3-PDO biosynthesis in microorganisms, and hopefully, it will provide reference for the renewable production of 1,3-PDO in industry.
1,3-丙二醇（1,3-PDO）是重要的有机化工原料之一，广泛应用于聚酯合成，在医药、化妆品、树脂和生物可降解塑料等领域也显示出巨大潜力。到目前为止，1,3-PDO主要来源于化学合成。然而，1,3-PDO化学合成过程中的副产物和副作用对环境造成了严重破坏。近年来，微生物中1,3-PDO的生物合成途径已被阐明。在甘油脱水酶（GDHt）和丙二醇氧化还原酶（PDOR）的作用下，甘油可通过还原途径被催化生成1,3-PDO。与化学合成相比，1,3-PDO的生物合成对环境友好，但会面临产量低的问题。为提高产量，已通过基因工程对天然的1,3-PDO生产菌株进行改造，并在模式微生物中重建了生物合成途径。在本综述中，我们总结了微生物中1,3-PDO生物合成的研究进展，希望能为工业上可再生生产1,3-PDO提供参考。
