Wang Yanxia, Wan Zijian, Zhu Yueting, Hu Haibo, Jiang Yujia, Jiang Wankui, Zhang Wenming, Xin Fengxue
College of Food Science and Light Industry, Nanjing Tech University, Nanjing, 211800, People's Republic of China.
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Puzhu South Road 30#, Nanjing, 211800, People's Republic of China.
Biotechnol Biofuels Bioprod. 2023 Mar 24;16(1):50. doi: 10.1186/s13068-023-02304-4.
1,3-Propanediol (1,3-PDO) is a platform compound, which has been widely used in food, pharmaceutical and cosmetic industries. Compared with chemical methods, the biological synthesis of 1,3-PDO has shown promising applications owing to its mild conditions and environmental friendliness. However, the biological synthesis of 1,3-PDO still has the problem of low titer and yield due to the shortage of reducing powers.
In this study, Klebsiella sp. strain YT7 was successfully isolated, which can synthesize 11.30 g/L of 1,3-PDO from glycerol in flasks. The intracellular redox regulation strategy based on the addition of electron mediators can increase the 1,3-PDO titer to 28.01 g/L. Furthermore, a co-culturing system consisting of strain YT7 and Shewanella oneidensis MR-1 was established, which can eliminate the supplementation of exogenous electron mediators and reduce the by-products accumulation. The 1,3-PDO yield reached 0.44 g/g and the final titer reached 62.90 g/L. The increased titer and yield were attributed to the increased redox levels and the consumption of by-products.
A two-bacterium co-culture system with Klebsiella sp. strain YT7 and S. oneidensis strain MR-1 was established, which realized the substitution of exogenous electron mediators and the reduction of by-product accumulation. Results provided theoretical basis for the high titer of 1,3-PDO production with low by-product concentration.
1,3 - 丙二醇（1,3 - PDO）是一种平台化合物，已广泛应用于食品、制药和化妆品行业。与化学方法相比，1,3 - PDO的生物合成因其温和的条件和环境友好性而显示出有前景的应用。然而，由于还原力的短缺，1,3 - PDO的生物合成仍然存在滴度和产量低的问题。
在本研究中，成功分离出克雷伯氏菌属YT7菌株，其在摇瓶中可从甘油合成11.30 g/L的1,3 - PDO。基于添加电子介质的细胞内氧化还原调节策略可将1,3 - PDO滴度提高到28.01 g/L。此外，建立了由YT7菌株和嗜铁还原地杆菌MR - 1组成的共培养系统，该系统可消除外源电子介质的添加并减少副产物积累。1,3 - PDO产量达到0.44 g/g，最终滴度达到62.90 g/L。滴度和产量的提高归因于氧化还原水平的提高和副产物的消耗。
建立了一种由克雷伯氏菌属YT7菌株和嗜铁还原地杆菌MR - 1组成的双菌共培养系统，该系统实现了外源电子介质的替代和副产物积累的减少。研究结果为高滴度、低副产物浓度生产1,3 - PDO提供了理论依据。
