Nxasana Thembelihle H, Mangoato Innocensia M, Masiu Patriciah M, Mishra Abhay P, Matsabisa Motlalepula G
Department of Pharmacology, University of the Free State, Faculty of Health Sciences, School of Medicine, Bloemfontein - South Africa.
Cosmetics and Natural Products Research Center (CosNat), Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok - Thailand.
Drug Target Insights. 2024 Dec 11;18:94-104. doi: 10.33393/dti.2024.3219. eCollection 2024 Jan-Dec.
Colorectal cancer incidences continue to increase annually, worldwide. Herbal plants with antiproliferative properties received research interest as agents that can be adjuvant therapies with chemotherapy drugs to enhance their efficacy and reverse drug resistance.
ethanolic (SBE) and aqueous (SBW) extracts combined with doxorubicin (DOX) against drug-sensitive and drug-resistant colorectal cancer cells were investigated for their potential adjuvant and drug resistance reversal. The extracts were assessed for their potential anticancer activities on HCT15 and HT29 cell lines as well as their doxorubicin potentiating and drug resistance reversal effects respectively. The extracts were assessed for their cytotoxicity on normal 3T3-L1 fibroblast cells.
Both SBE and SBW extracts exhibited no toxicity against normal 3T3 cells and showed low activity on the HT29 cell line. Contrarily, resistant HCT15 cells showed moderate to low activity with significantly higher inhibitory concentration (IC) values. The combination of SBE with DOX and SBW with DOX resulted in antagonistic interactions, causing an increase in IC values for HT29 and HCT15 cells. In contrast, the combination of DOX and verapamil (VER) produced an additive effect, with no change in their IC values.
Based on the findings from the combination treatment, the SBE and SBW extracts demonstrated higher efficacy and synergistic effects combined with DOX at IC compared to the combination of DOX and VER, suggesting their potential as anticancer agents. However, further research on both the SBE and SBW extracts' mechanisms of action and in vivo effects is warranted.
在全球范围内，结直肠癌的发病率逐年持续上升。具有抗增殖特性的草药作为辅助化疗药物以增强其疗效和逆转耐药性的药物而受到研究关注。
研究了乙醇提取物（SBE）和水提取物（SBW）与阿霉素（DOX）联合作用于对药物敏感和耐药的结直肠癌细胞的潜在辅助作用及耐药逆转作用。分别评估了这些提取物对HCT15和HT29细胞系的潜在抗癌活性及其对阿霉素的增效作用和耐药逆转作用。还评估了这些提取物对正常3T3-L1成纤维细胞的细胞毒性。
SBE和SBW提取物对正常3T3细胞均无毒性，对HT29细胞系的活性较低。相反，耐药的HCT15细胞表现出中度至低度活性，其抑制浓度（IC）值明显更高。SBE与DOX以及SBW与DOX的组合产生拮抗作用，导致HT29和HCT15细胞的IC值增加。相比之下，DOX与维拉帕米（VER）的组合产生相加作用，其IC值没有变化。
基于联合治疗的结果，与DOX和VER的组合相比，SBE和SBW提取物与DOX联合在IC时显示出更高的疗效和协同作用，表明它们具有作为抗癌剂的潜力。然而，有必要对SBE和SBW提取物的作用机制及体内效应进行进一步研究。
