巨噬细胞与不孕症：抗精子抗体增强人类巨噬细胞介导的精子杀伤作用

Macrophages and infertility: enhancement of human macrophage-mediated sperm killing by antisperm antibodies.

作者信息

London S N, Haney A F, Weinberg J B

出版信息

Fertil Steril. 1985 Feb;43(2):274-8.

Abstract

The mechanism by which antisperm antibodies inhibit fertility is not completely understood. Macrophages may play a role in mediating infertility by interacting with sperm and destroying gametes. Experiments were conducted evaluating the effect of antisperm antibody on the phagocytosis and lysis of sperm by human peritoneal macrophages in vitro. Sperm from a fertile man treated with sera from normal men and women or medium alone had 5 to 280 molecules of IgG/sperm, as determined by a 125I-labeled anti-human IgG monoclonal antibody assay. By contrast, sperm treated with sera containing antisperm antibodies had 310 to 1240 molecules of IgG/sperm. Peritoneal macrophages harvested from infertile women with tubal/adhesive problems mediated phagocytosis and lysis of 111In-labeled sperm which was enhanced by treatment of the sperm with sera containing antisperm antibodies (39.0% +/- 1.5% versus 76.3% +/- 3.2% phagocytosis, and 3.3% +/- 0.3% versus 23.3% +/- 2.3% lysis of sperm [control versus antibody-treated]). The likelihood of fertilization in couples with antisperm antibody may be determined not only by the antibody but also by the presence of genital tract macrophages capable of destroying the antibody-coated sperm.

摘要

抗精子抗体抑制生育能力的机制尚未完全明确。巨噬细胞可能通过与精子相互作用并破坏配子，在介导不孕方面发挥作用。进行了实验，以评估抗精子抗体对人腹膜巨噬细胞体外吞噬和裂解精子的影响。通过125I标记的抗人IgG单克隆抗体测定法确定，用正常男性和女性的血清或仅用培养基处理的可育男性的精子，每个精子有5至280个IgG分子。相比之下，用含抗精子抗体的血清处理的精子，每个精子有310至1240个IgG分子。从患有输卵管/粘连问题的不孕女性中采集的腹膜巨噬细胞介导了111In标记精子的吞噬和裂解，用含抗精子抗体的血清处理精子可增强这种作用（吞噬率分别为39.0%±1.5%和76.3%±3.2%，精子裂解率分别为3.3%±0.3%和23.3%±2.3%[对照与抗体处理]）。有抗精子抗体的夫妇的受精可能性不仅可能取决于抗体，还可能取决于是否存在能够破坏被抗体包被精子的生殖道巨噬细胞。