Seco-Hidalgo Victor, Witney Adam, Chico Martha E, Vaca Maritza, Arevalo Andrea, Schuyler Alexander J, Platts-Mills Thomas Ae, Ster Irina Chis, Cooper Philip J
St George's University of London.
Fundación Ecuatoriana Para la Investigación en Salud.
Res Sq. 2024 Dec 2:rs.3.rs-5361957. doi: 10.21203/rs.3.rs-5361957/v1.
There are limited longitudinal data from non-industrialized settings on patterns and determinants of gut bacterial microbiota development in early childhood. We analysed epidemiological data and stool samples collected from 60 children followed from early infancy to 5 years of age in a rural tropical district in coastal Ecuador. Data were collected longitudinally on a wide variety of individual, maternal, and household exposures. Extracted DNA from stool samples were analyzed for bacterial microbiota using 16S rRNA gene sequencing. Both alpha and beta diversity indices suggested stable profiles towards 5 years of age. Greater alpha diversity and lower beta diversity were associated with factors typical of rural poverty including low household incomes, overcrowding, and greater agricultural and animal exposures, but not with birth mode or antibiotic exposures. Consumption of unpasteurized milk was consistently associated with greater alpha diversity indices. Infants living in a non-industrialized setting in conditions of greater poverty and typically rural exposures appeared to acquire more rapidly a stable and diverse gut bacterial microbiome during childhood.
关于非工业化环境中幼儿肠道细菌微生物群发育模式和决定因素的纵向数据有限。我们分析了从厄瓜多尔沿海一个热带农村地区60名儿童收集的流行病学数据和粪便样本，这些儿童从婴儿早期一直追踪到5岁。纵向收集了关于各种个人、母亲和家庭暴露情况的数据。使用16S rRNA基因测序分析从粪便样本中提取的DNA的细菌微生物群。α和β多样性指数均表明，到5岁时微生物群特征稳定。较高的α多样性和较低的β多样性与农村贫困的典型因素有关，包括家庭收入低、过度拥挤以及更多接触农业和动物，但与出生方式或抗生素暴露无关。食用未杀菌牛奶始终与较高的α多样性指数相关。生活在非工业化环境中、贫困程度较高且通常有农村暴露情况的婴儿，在童年时期似乎能更快地获得稳定且多样的肠道细菌微生物群。
