Verma Nandini, Riyaz Mohammad, Kaur Gurkeerat, Negi Preeti, Ghawri Harshita, Raj Khem
Department of Microbiology, Basic Medical Sciences Block 1, Panjab University, South Campus, Sector-25, Chandigarh, 160014 India.
Present Address: MTCC Gene Bank, CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh, 160036 India.
Indian J Microbiol. 2024 Dec;64(4):1912-1928. doi: 10.1007/s12088-024-01277-8. Epub 2024 Apr 28.
is the most common, globally detected nosocomial fungal pathogen with multi-drug resistance. The high prevalence of infections has raised concern about drug resistance and adverse effects, compounded by a lack of effective alternative drugs. Bioengineered nanomaterials play a significant role in combating nosocomial infections. Silver nanoparticles (AgNPs) have emerged as an extensively used nanomaterial due to their prominent antimicrobial properties. One of the most promising approaches is to incorporate herbal extracts that contain a range of phytoconstituents, being used for curing various chronic illnesses. This study aimed to produce eco-friendly, cost-effective green synthesized AgNPs with trans-Himalayan medicinal plant extracts ( & ) and assess their anticandidal and antibiofilm potential. The green-synthesized AgNPs formation and crystalline nature were confirmed by UV-visible spectroscopy, dynamic light scattering and X-ray diffraction analysis. The UV-Vis spectra of the AgNPs revealed bands in the range of 415-430 nm. Phytoconstituents as reducing agents were involved in the stabilization of AgNPs as identified by FTIR spectra. HR-TEM of AgNPs' displayed a spherical shape with size in the range of 10-100 nm. Results of activity tests performed using various clinical strains showed half maximum growth inhibition (IC) at 8.02 µg/mL, which inhibited 65% of biofilm for extract. The free radical scavenging activity evaluated for green synthesized AgNPs using DPPH showed more than 90% antioxidant activity. Green synthesized AgNPs displayed potent growth inhibition (IC) at 4.01 µg/mL with 87.0% biofilm inhibition. Green synthesized AgNPs coated bandages and catheters inhibited the growth of . This study concluded that green synthesized AgNPs formulation in conjunction with antifungal agents exhibits potential biomedical application and also could be used as alternative therapeutics.
The online version contains supplementary material available at 10.1007/s12088-024-01277-8.
是全球检测到的最常见的具有多重耐药性的医院内真菌病原体。感染的高发生率引发了对耐药性和不良反应的担忧，而缺乏有效的替代药物则使情况更加复杂。生物工程纳米材料在对抗医院感染方面发挥着重要作用。银纳米颗粒（AgNPs）因其突出的抗菌特性而成为广泛使用的纳米材料。最有前景的方法之一是加入含有一系列植物成分的草药提取物，这些提取物被用于治疗各种慢性疾病。本研究旨在利用喜马拉雅药用植物提取物（&）制备环保、经济高效的绿色合成AgNPs，并评估其抗念珠菌和抗生物膜潜力。通过紫外可见光谱、动态光散射和X射线衍射分析确认了绿色合成AgNPs的形成和晶体性质。AgNPs的紫外可见光谱显示在415 - 430nm范围内有吸收带。通过傅里叶变换红外光谱确定，植物成分作为还原剂参与了AgNPs的稳定化。AgNPs的高分辨率透射电子显微镜显示为球形，尺寸在10 - 100nm范围内。使用各种临床菌株进行的活性测试结果表明，在8.02µg/mL时达到半数最大生长抑制（IC），对提取物而言，该浓度抑制了65%的生物膜形成。使用二苯基苦味酰基自由基（DPPH）对绿色合成AgNPs进行的自由基清除活性评估显示，其抗氧化活性超过90%。绿色合成AgNPs在4.01µg/mL时显示出强大的生长抑制（IC），生物膜抑制率为87.0%。绿色合成AgNPs涂层绷带和导管抑制了的生长。本研究得出结论，绿色合成AgNPs制剂与抗真菌剂联合使用具有潜在的生物医学应用价值，也可作为替代疗法。
在线版本包含可在10.1007/s12088-024-01277-8获取的补充材料。
