Rockwell S
Int J Radiat Oncol Biol Phys. 1985 Jan;11(1):97-103. doi: 10.1016/0360-3016(85)90367-0.
The ability of the perfluorochemical emulsion, Fluosol-DA, to oxygenate the naturally-occurring hypoxic cells of solid tumors was examined. BALB/c mice bearing EMT6 mouse mammary tumors were given in a single i.v. injection of 0.015 ml/g of Fluosol, and were then treated with 95% O2/5% CO2 for 30 min before irradiation with graded doses of X rays. The pretreatment increased the cytotoxic effect of the radiation, as assayed by colony formation in vitro. The dose-response curve for the combination regimen was parallel to that for radiation alone, but was offset towards lower survivals. This change is consistent with the change which would be expected if the pretreatment decreased the proportion of hypoxic cells in the tumors. Studies with unoxygenated perfluorochemical emulsions in vivo and in vitro and studies with O2/CO2 alone in vivo suggested that the survival curve changes did not reflect either direct cytotoxic effects of the perfluorochemical emulsion or the O2/CO2-breathing, or radiosensitizing or physiologic effects of either agent alone. The data reported here suggest that oxygenated perfluorochemical emulsions may decrease the proportion of hypoxic cells in solid animal tumors and encourage further preclinical evaluation of these agents as potential adjuvants to radiotherapy.
研究了全氟化合物乳液Fluosol-DA对实体瘤中自然存在的缺氧细胞进行氧合的能力。给患有EMT6小鼠乳腺肿瘤的BALB/c小鼠静脉注射0.015 ml/g的Fluosol，然后在接受不同剂量的X射线照射前，用95% O2/5% CO2处理30分钟。如通过体外集落形成所测定的，预处理增强了辐射的细胞毒性作用。联合治疗方案的剂量反应曲线与单独放疗的曲线平行，但向较低生存率偏移。这种变化与如果预处理降低了肿瘤中缺氧细胞比例所预期的变化一致。体内和体外使用未氧合全氟化合物乳液的研究以及体内单独使用O2/CO2的研究表明，生存曲线的变化既不反映全氟化合物乳液的直接细胞毒性作用，也不反映O2/CO2呼吸作用，也不反映单独任何一种药物的放射增敏或生理作用。此处报告的数据表明，氧合全氟化合物乳液可能会降低实体动物肿瘤中缺氧细胞的比例，并鼓励对这些药物作为放疗潜在佐剂进行进一步的临床前评估。
