Li Xiaoyang, Yan Yufei, Hu Chenyi, Wang Jing, Wang Jinlin, Yang Hao, Cui Daxiang, Xin Wenwen, Gao Shan, Jin Han
State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing, 100071, China.
Institute of Micro-Nano Science and Technology & National Key Laboratory of Advanced Micro and Nano Manufacture Technology, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai, 200240, P. R. China.
Adv Sci (Weinh). 2025 Feb;12(6):e2411738. doi: 10.1002/advs.202411738. Epub 2024 Dec 16.
Accurately assessing potential side effects following botulinum neurotoxin (BoNT) injection remains a formidable challenge. To address this issue, an innovative approach is developed that combines a wearable temperature sensor with a sophisticated volatilomics technique, aimed at facilitating the rapid and convenient prediction of potential physical discomfort related to latrogenic botulism. The investigation identifies five volatile organic compounds (VOCs)-acetone, styrene, ethanol, 2-pentanone, and n-butano-as promising markers indicative of BoNT poisoning. Specifically, a handheld breath analyzer, featuring a yttrium stabilized zirconia (YSZ)-based gas sensor array, alongside a wearable temperature sensor integrated with a bio-compatible methacrylated gelatin (GelMA) sensing film, are developed to simultaneously monitor breath signal variations and body temperature fluctuations. Preliminary animal testing validates the effectiveness of the integrated approach, achieving an accuracy exceeding 91.2% in early detection of physical discomfort associated with BoNT poisoning. These promising findings represent a significant advancement towards the early identification of BoNT-related issues, enabling timely intervention and improved management strategies.
准确评估肉毒杆菌神经毒素（BoNT）注射后的潜在副作用仍然是一项艰巨的挑战。为了解决这个问题，开发了一种创新方法，将可穿戴温度传感器与先进的挥发物组学技术相结合，旨在促进对与医源性肉毒中毒相关的潜在身体不适进行快速便捷的预测。该研究确定了五种挥发性有机化合物（VOCs）——丙酮、苯乙烯、乙醇、2-戊酮和正丁烷——作为指示BoNT中毒的有前景的标志物。具体而言，开发了一种手持式呼吸分析仪，其具有基于钇稳定氧化锆（YSZ）的气体传感器阵列，以及与生物相容性甲基丙烯酸化明胶（GelMA）传感膜集成的可穿戴温度传感器，以同时监测呼吸信号变化和体温波动。初步动物试验验证了该综合方法的有效性，在早期检测与BoNT中毒相关的身体不适方面，准确率超过91.2%。这些有前景的发现代表了在早期识别与BoNT相关问题方面的重大进展，能够实现及时干预并改善管理策略。
