Fujita Hideaki, Watanabe Tomonobu M
Department of Stem Cell Biology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Minami-ku, Hiroshima 734-0037, Japan.
Laboratory for Comprehensive Bioimaging, RIKEN Center for Biosystems Dynamics Research (BDR), Chuo-ku, Kobe 650-0047, Japan.
J Radiat Res. 2024 Dec 16;65(Supplement_1):i117-i125. doi: 10.1093/jrr/rrae016.
Radiation induces various changes in biological specimens; however, the evaluation of these changes is usually complicated and can be achieved only through investment in time and labor. Optical methods reduce the cost of such evaluations as they require less pretreatment of the sample, are adaptable to high-throughput screening and are easy to automate. Optical methods are also advantageous, owing to their real-time and onsite evaluation capabilities. Here, we discuss three optical technologies to evaluate the effects of radiation on biological samples: single-molecule tracking microscopy to evaluate the changes in the physical properties of DNA, Raman spectral microscopy for dosimetry using human hair and second-harmonic generation microscopy to evaluate the effect of radiation on the differentiation of stem cells. These technologies can also be combined for more detailed information and are applicable to other biological samples. Although optical methods are not commonly used to evaluate the effects of radiation, advances in this technology may facilitate the easy and rapid assessment of radiation effects on biological samples.
辐射会在生物样本中引发各种变化；然而，对这些变化的评估通常很复杂，且只有通过投入时间和人力才能实现。光学方法降低了此类评估的成本，因为它们对样本的预处理要求较低，适用于高通量筛选且易于自动化。光学方法还因其具有实时和现场评估能力而具有优势。在此，我们讨论三种用于评估辐射对生物样本影响的光学技术：用于评估DNA物理性质变化的单分子追踪显微镜、利用人发来进行剂量测定的拉曼光谱显微镜以及用于评估辐射对干细胞分化影响的二次谐波产生显微镜。这些技术也可以结合起来以获取更详细的信息，并且适用于其他生物样本。尽管光学方法并不常用于评估辐射的影响，但该技术的进步可能会促进对生物样本辐射影响的轻松快速评估。
