菊欧文氏菌己糖醛酸降解缺陷型突变体的分离与鉴定
Isolation and characterization of Erwinia chrysanthemi mutants defective in degradation of hexuronates.
作者信息
van Gijsegem F, Hugouvieux-Cotte-Pattat N, Robert-Baudouy J
出版信息
J Bacteriol. 1985 Feb;161(2):702-8. doi: 10.1128/jb.161.2.702-708.1985.
Abstract
Spontaneous and Tn9-induced mutants of Erwinia chrysanthemi were isolated which affect the degradative pathway of galacturonate and ketodeoxygluconate. The mutations were characterized both biochemically and functionally by complementation analysis and localized in the E. chrysanthemi chromosome. The kdgK gene mapped very close to ile, the kdgA gene was between trp and his, and the exuT-uxaC-uxaB-uxaA cluster was linked to thy. The different types of mutants obtained were consistent with an organization of the exu-uxa cluster into two transcription units, one containing the exuT gene, and the other containing the three uxa genes, with the transcription going from uxaC to uxaA.
摘要
分离出了菊欧文氏菌的自发突变体和Tn9诱导突变体，这些突变体影响半乳糖醛酸和酮脱氧葡萄糖酸的降解途径。通过互补分析对这些突变体进行了生化和功能表征，并定位在菊欧文氏菌染色体上。kdgK基因定位于非常靠近ile的位置，kdgA基因位于trp和his之间，exuT-uxaC-uxaB-uxaA簇与thy相连。获得的不同类型突变体与exu-uxa簇组织成两个转录单位一致，一个包含exuT基因，另一个包含三个uxa基因，转录从uxaC到uxaA。
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