大肠杆菌K-12中己糖醛酸系统基因的调控：exuR和uxuR基因产物对uxu操纵子的多重调控
Regulation of hexuronate system genes in Escherichia coli K-12: multiple regulation of the uxu operon by exuR and uxuR gene products.
作者信息
Robert-Baudouy J, Portalier R, Stoeber F
出版信息
J Bacteriol. 1981 Jan;145(1):211-20. doi: 10.1128/jb.145.1.211-220.1981.
Abstract
New regulatory mutants of Escherichia coli K-1 carrying alterations of the uxuR gene were isolated and characterized. In the presence of superrepressed or derepressed uxuR mutations, mannonic hydrolyase (uxuA) and oxidoreductase relationship analyses suggested that the uxuR gene product acted as a repressor in the control of uxuA-uxuB operon expression. uxuR mutations were localized near min 97, and the following gene order was established: (argH)-uxuR-uxuB-uxuA-(thr). Properties of exuR (point and deletion) mutants showed that both exuR and uxuR regulatory gene products were involved in the control of the uxuA uxuB operon. Analysis of exuR uxuR double-derepressed mutants suggested that exuR and uxuR repressors act cooperatively to repress the uxu operon.
摘要
分离并鉴定了携带uxuR基因改变的大肠杆菌K-1新调控突变体。在存在超阻遏或去阻遏的uxuR突变的情况下，甘露糖水解酶（uxuA）和氧化还原酶关系分析表明，uxuR基因产物在uxuA-uxuB操纵子表达的控制中起阻遏作用。uxuR突变定位在约97分钟处，并确定了以下基因顺序：（argH）-uxuR-uxuB-uxuA-（thr）。exuR（点突变和缺失）突变体的特性表明，exuR和uxuR调控基因产物都参与了uxuA uxuB操纵子的控制。对exuR uxuR双去阻遏突变体的分析表明，exuR和uxuR阻遏物协同作用以阻遏uxu操纵子。
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