Bognár László, Kőrösi Zsolt Jenő, Bene Szabolcs Albin, Szabó Ferenc, Anton István, Zsolnai Attila
Association of Hungarian Holstein Breeders, Lőportár utca 16., H-1134 Budapest, Hungary.
Albert Kázmér Faculty of Mosonmagyaróvár, Széchenyi István University, Vár tér 2., H-9200 Mosonmagyaróvár, Hungary.
Animals (Basel). 2024 Dec 5;14(23):3518. doi: 10.3390/ani14233518.
This study aimed to find SNPs that have an effect on the estimated breeding values (EBVs) of milk (MY), fat (FY), and protein yield (PY) of Holstein Friesian cows in Hungary. Holstein Friesian cows (n = 2963) were genotyped on a Eurogenomics (EuroG_MDv4) chip. The EBVs for MY, FY, and PY were obtained from the Association of Hungarian Holstein Breeders (AHHB). The loci associated with the EBVs were identified via three approaches: the calculation of genetic distance of the SNPs (), linear regression, and haplotype association tests. Nine SNPs were significantly associated with MY, FY, and PY located on BTA 2, 5, 28, and X. Among the nine SNPs identified, BTB-00219372 on BTA 5 had a positive β coefficient for MY and a negative β coefficient for FY and PY. In addition, BovineHD3000027615 on BTA X had a positive β coefficient for both MY and PY, as well as a negative β coefficient for FY. The identified SNPs were located near several genes that remain unstudied in cattle, which are potential targets for closer scrutiny in relation to milk properties. The markers associated with two or three EBVs could be used in selection with high efficiency to accelerate genetic development and help AHHB experts achieve their breeding. Most marker effects point in the same direction on EBVs; however, we found that BTB-00219372 and BovineHD3000027615 could be used with caution to increase one EBV while decreasing the other EBV or EBVs.
本研究旨在寻找对匈牙利荷斯坦弗里生奶牛的牛奶产量（MY）、脂肪产量（FY）和蛋白质产量（PY）的估计育种值（EBV）有影响的单核苷酸多态性（SNP）。对2963头荷斯坦弗里生奶牛进行了欧洲基因组学（EuroG_MDv4）芯片基因分型。MY、FY和PY的EBV数据来自匈牙利荷斯坦育种者协会（AHHB）。通过三种方法确定与EBV相关的基因座：计算SNP的遗传距离、线性回归和单倍型关联测试。在BTA 2、5、28和X染色体上发现9个SNP与MY、FY和PY显著相关。在鉴定出的9个SNP中，BTA 5上的BTB-00219372对MY的β系数为正，对FY和PY的β系数为负。此外，BTA X上的BovineHD3000027615对MY和PY的β系数均为正，对FY的β系数为负。所鉴定的SNP位于牛中尚未研究的几个基因附近，这些基因是与牛奶特性相关的更深入研究的潜在目标。与两个或三个EBV相关的标记可高效用于选择，以加速遗传进展，并帮助AHHB的专家实现他们的育种目标。大多数标记对EBV的影响方向相同；然而，我们发现BTB-00219372和BovineHD3000027615在使用时需谨慎，因为它们可能在增加一个EBV的同时降低另一个或其他EBV。
