Karuppusamy Shanmugapriya, Wanigasekara Janith, Fitzpatrick Stephen, Lyons Henry, Curtin James, Rajauria Gaurav, Tiwari Brijesh K, O'Donnell Colm
School of Biosystems and Food Engineering, University College Dublin, D04 V1W8 Dublin, Ireland.
School of Food Science and Environmental Health, College of Sciences and Health, Technological University Dublin, D01 K822 Dublin, Ireland.
Cells. 2024 Nov 22;13(23):1938. doi: 10.3390/cells13231938.
This study aimed to investigate the biological activity of crude and purified laminarin and fucoidan samples extracted from Irish brown macroalgae species and . The antioxidant capacity of the samples was evaluated using the 2,2-diphenyl-1-picrylhydrazyl and ferric-reducing antioxidant power assays. The anti-inflammatory potential of the samples was analysed using the cyclooxygenases inhibition activity, and the antidiabetic activity was evaluated using a dipeptidyl peptidase-4 inhibitor screening assay. The cytotoxicity of the samples was measured using the Alamar Blue™ assay with different types of cancer cell lines. The crude laminarin and fucoidan samples exhibited higher antioxidant activity ( < 0.05) than the purified samples and commercial standards. Similarly, the crude extracts showed stronger anti-inflammatory and antidiabetic effects compared to the purified samples. Additionally, the crude laminarin and fucoidan samples showed higher cytotoxic activity. Specifically, as confirmed in the flow cytometry analysis, 3D tumour spheres using different cancer cell lines showed significantly higher resistance to bioactive compounds compared to 2D monolayer cells. The laminarin and fucoidan polysaccharide samples investigated are suitable for potential nutraceutical applications based on the biological activity values observed. Future research is necessary to purify the bioactive compounds investigated and improve their selectivity for targeted therapeutic uses in food and biomedical applications.
本研究旨在调查从爱尔兰褐藻物种和中提取的粗制及纯化海带多糖和岩藻聚糖样品的生物活性。使用2,2-二苯基-1-苦基肼自由基和铁还原抗氧化能力测定法评估样品的抗氧化能力。使用环氧化酶抑制活性分析样品的抗炎潜力，并使用二肽基肽酶-4抑制剂筛选测定法评估抗糖尿病活性。使用Alamar Blue™测定法对不同类型的癌细胞系测量样品的细胞毒性。粗制海带多糖和岩藻聚糖样品比纯化样品和商业标准品表现出更高的抗氧化活性（<0.05）。同样，与纯化样品相比，粗提物显示出更强的抗炎和抗糖尿病作用。此外，粗制海带多糖和岩藻聚糖样品显示出更高的细胞毒性活性。具体而言，如流式细胞术分析所证实的，与二维单层细胞相比，使用不同癌细胞系的三维肿瘤球对生物活性化合物表现出显著更高的抗性。基于观察到的生物活性值，所研究的海带多糖和岩藻聚糖多糖样品适用于潜在的营养保健应用。有必要开展进一步研究以纯化所研究的生物活性化合物，并提高其在食品和生物医学应用中靶向治疗用途的选择性。
