Emenike Chijioke, Azeez Shakirah, English Marcia, Udenigwe Chibuike, Abbey Lord
Department of Plant, Food, and Environmental Sciences, Faculty of Agriculture, Dalhousie University, Truro, NS B2N5E3, Canada.
Faculty of Natural and Applied Sciences, Hezekiah University, Umudi, Nkwerre 471115, Nigeria.
Foods. 2024 Nov 24;13(23):3762. doi: 10.3390/foods13233762.
Haskap ( L.) has gained much research interest, given the diverse biologically active compounds found in different parts of the plant. It is, therefore, important to study the concentration of some of these biologically active compounds at different developmental stages. The present study investigated the effect of growth stages on nutrients, metabolites, and polyphenol concentrations in the leaves of three haskap varieties (Indigo Gem, Wojtek, and Jolanta). A targeted quantitative metabolomics approach was used to analyze the haskap leaves using a combination of direct injection mass spectrometry with a reverse LC-MS/MS custom assay. The results showed that the concentrations of soluble sugar, organic acids, polyphenols, and amino acids in the leaves of different varieties of haskap were decreased at the fruiting stage. The leafing stage may be the best period to harvest haskap leaves with the highest nutrients and polyphenol concentrations. Studies on polyphenols and nutrient characterization of haskap leaf at the leafing stage could be further investigated.
由于在哈斯卡普（L.）植物的不同部位发现了多种生物活性化合物，它已引起了广泛的研究兴趣。因此，研究其中一些生物活性化合物在不同发育阶段的浓度很重要。本研究调查了生长阶段对三个哈斯卡普品种（靛蓝宝石、沃伊捷克和乔兰塔）叶片中营养成分、代谢物和多酚浓度的影响。采用靶向定量代谢组学方法，结合直接进样质谱和反向LC-MS/MS定制分析方法对哈斯卡普叶片进行分析。结果表明，不同品种哈斯卡普叶片中的可溶性糖、有机酸、多酚和氨基酸浓度在结果期均有所下降。展叶期可能是收获营养成分和多酚浓度最高的哈斯卡普叶片的最佳时期。可以进一步研究展叶期哈斯卡普叶片的多酚和营养特性。
