Department of Pharmacognosy, Faculty of Pharmacy, Poznań University of Medical Sciences, 60-806 Poznan, Poland.
Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, 60-627 Poznan, Poland.
Nutrients. 2022 Sep 21;14(19):3898. doi: 10.3390/nu14193898.
The presented research evaluates the medical use potential of leaves, which are waste plants in cultivating berries. The study's screening activity included the leaves of five varieties of : Atut, Duet, Wojtek, Zojka, and Jugana. The microbiological analysis confirmed the safety of using leaves without significant stabilization. leaves standardization was carried out based on the results of the chromatographic analysis, and it showed differences in the contents of active compounds (loganic, chlorogenic and caffeic acids, and rutin), which are attributed to biological activity. For the leaves varieties tested, the differences in the content of total polyphenol content, chlorophylls, and carotenoids were also confirmed. The screening of biological activity of five leaf varieties was carried out concerning the possibility of inhibiting the activity of α-glucosidase, lipase, and hyaluronidase as well, and the antioxidant potential was determined. The defined profile of the biological activity of leaves makes it possible to indicate this raw material as an essential material supporting the prevention and treatment of type II diabetes. However, this research showed that tested enzymes were strongly inhibited by the variety Jugana. The health-promoting potential of leaves was correlated with the highest chlorogenic acid and rutin content in the variety Jugana.
本研究评估了在浆果种植中作为废料的 叶的医学应用潜力。研究的筛选活动包括五种品种的 叶：Atut、Duet、Wojtek、Zojka 和 Jugana。微生物分析证实了使用 叶而无需显著稳定的安全性。根据色谱分析的结果对 叶进行了标准化，结果表明活性化合物（龙胆酸、绿原酸和咖啡酸以及芦丁）的含量存在差异，这归因于其生物活性。对于所测试的 叶品种，还证实了总多酚含量、叶绿素和类胡萝卜素的含量存在差异。对五种 叶品种的生物活性进行了筛选，以研究其抑制α-葡萄糖苷酶、脂肪酶和透明质酸酶活性的可能性，并测定了其抗氧化潜力。 叶的明确生物活性特征使其有可能被指定为支持 II 型糖尿病预防和治疗的重要原料。然而，这项研究表明，测试的酶被 Jugana 品种强烈抑制。 叶的促进健康潜力与其在 Jugana 品种中最高的绿原酸和芦丁含量相关。
