García-Juárez Adamaris, Garzón-García Alba Mery, Ramos-Enríquez José Rogelio, Tapia-Hernández José Agustín, Ruiz-Cruz Saúl, Canizales-Rodríguez Dalila Fernanda, Del-Toro-Sánchez Carmen Lizette, Rodríguez-Félix Francisco, Ocaño-Higuera Víctor Manuel, Ornelas-Paz José de Jesús
Departamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Encinas y Rosales s/n, Hermosillo 83000, Sonora, Mexico.
Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Encinas y Rosales s/n, Hermosillo 83000, Sonora, Mexico.
Foods. 2024 Nov 28;13(23):3838. doi: 10.3390/foods13233838.
Bitter orange is a citrus fruit rich in bioactive compounds, but its waste is currently underutilized. One potential solution is to encapsulate these bioactive compounds. This research aims to synthesize gelatin nanoparticles loaded with an ethanolic extract of bitter orange peel and to evaluate their in vitro antioxidant and antibacterial activities. Coaxial electrospray was used to encapsulate the ethanolic extract of bitter orange with bovine gelatin as wall material, considering a voltage of 15 kV, a wall solution flow rate of 0.1 mL/h, and a core solution flow rate of 0.08 mL/h. Characterization of the nanoparticles was performed using scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR). Antioxidant activity was evaluated by the total phenolic content, flavonoids, and antioxidant capacity by the DPPH, ABTS, and FRAP assays. Antibacterial activity was assessed by the well diffusion technique on Mueller-Hinton agar against and O157:H7 bacteria. SEM images confirmed that the nanoparticles were spherical in shape, while FT-IR analysis indicated that the incorporation of the extract did not alter the amide bonds of the gelatin protein. The nanoparticles containing the extract exhibited higher antioxidant activity and heightened inhibition against O157:H7, indicating their potential food applications.
酸橙是一种富含生物活性化合物的柑橘类水果，但其废弃物目前未得到充分利用。一种潜在的解决方案是将这些生物活性化合物进行包封。本研究旨在合成负载酸橙皮乙醇提取物的明胶纳米颗粒，并评估其体外抗氧化和抗菌活性。采用同轴电喷雾法，以牛明胶为壁材包封酸橙乙醇提取物，电压为15 kV，壁材溶液流速为0.1 mL/h，芯材溶液流速为0.08 mL/h。使用扫描电子显微镜（SEM）和傅里叶变换红外光谱（FT-IR）对纳米颗粒进行表征。通过总酚含量、黄酮类化合物以及DPPH、ABTS和FRAP法测定抗氧化能力来评估抗氧化活性。通过在Mueller-Hinton琼脂上的打孔扩散技术评估对金黄色葡萄球菌和O157:H7细菌的抗菌活性。SEM图像证实纳米颗粒呈球形，而FT-IR分析表明提取物的掺入未改变明胶蛋白的酰胺键。含有提取物的纳米颗粒表现出更高的抗氧化活性以及对O157:H7更强的抑制作用，表明其在食品领域的应用潜力。
