Iacovelli Riccardo, Poon Fons, Haslinger Kristina
Department of Chemical and Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, 9713 AV Groningen, The Netherlands.
Molecules. 2024 Nov 26;29(23):5576. doi: 10.3390/molecules29235576.
(Lion's Mane mushroom) is widely consumed for its numerous reported benefits for brain health. A growing body of evidence suggests that these benefits are likely attributable to aromatic compounds contained in its fruiting bodies, including the meroterpenoids hericenones. Here, we report the identification and reconstitution of the first two steps of the biosynthetic pathway of hericenones via heterologous expression of the polyketide synthase HerA and the carboxylic acid reductase HerB in . Furthermore, we investigated a putative prenyltransferase that might be responsible for the following biosynthetic step. Ongoing efforts to reconstitute the full pathway will enable large-scale production of hericenones and other meroterpenoids in heterologous hosts.
（猴头菇）因其对大脑健康有诸多益处而被广泛食用。越来越多的证据表明，这些益处可能归因于其子实体中含有的芳香化合物，包括半萜类化合物猴头菌素。在此，我们报告了通过聚酮合酶HerA和羧酸还原酶HerB在[具体宿主]中的异源表达，鉴定并重构了猴头菌素生物合成途径的前两步。此外，我们研究了一种可能负责后续生物合成步骤的假定异戊烯基转移酶。正在进行的重构完整途径的努力将使在异源宿主中大规模生产猴头菌素和其他半萜类化合物成为可能。
