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作为一种环境管理方法从实际废水中去除病毒

Virus Removal from Real Wastewater as an Environmental Management Approach.

作者信息

Gomes João, Domingues Eva, Frasson Danilo, Martins Rui C, Matos Ana Miguel

机构信息

CERES, Department of Chemical Engineering, Faculty of Sciences and Technology, University of Coimbra, Rua Sílvio Lima, 3030-790 Coimbra, Portugal.

CERES, Microbiology Laboratory, Faculty of Pharmacy, University of Coimbra, Pólo das Ciências da Saúde, 3000-548 Coimbra, Portugal.

出版信息

Molecules. 2024 Nov 27;29(23):5601. doi: 10.3390/molecules29235601.

Abstract

The increased presence of resistant microorganisms in water promotes the need for supplementary measures to mitigate the water source's contamination. Traditional treatments are inefficient in wastewater management at removing some emerging contaminants. an invasive species, can be used in the treatment due to their resistance and biofiltration capacity, working as a pest management strategy. In this study, this bivalve was used to promote the virus disinfection from the municipal wastewater treatment plant (MWTP) that enters (influent) and after the secondary treatment (effluent leaving the plant). JC virus, norovirus (GI, GII), and hepatitis A (HAV) were identified. promoted norovirus GI and GII removal after 72 h and a slight decrease in the JC virus concentration. These results prove the potential of this pest management approach to be used in virus removal. Furthermore, infectivity assays using mengovirus confirmed the correlation between the presence of the genome detected by PCR and the infectious virus particles. This highlights the potential of PCR as a reliable indicator of the infectious virus's presence. However, such an infectivity assay proved that even when PCR results are undetectable, a reduced number of viruses may remain viable and able to infect susceptible cells in culture.

摘要

水中耐药微生物的增加促使人们需要采取补充措施来减轻水源污染。传统处理方法在去除一些新兴污染物的废水管理中效率低下。一种入侵物种,因其抗性和生物过滤能力,可用于处理,作为一种害虫管理策略。在本研究中,这种双壳贝类被用于促进城市污水处理厂(MWTP)进水(流入)和二级处理后(流出工厂的出水)中病毒的消毒。鉴定出了JC病毒、诺如病毒(GI、GII)和甲型肝炎病毒(HAV)。72小时后促进了诺如病毒GI和GII的去除,JC病毒浓度略有下降。这些结果证明了这种害虫管理方法在去除病毒方面的潜力。此外,使用脑心肌炎病毒的感染性测定证实了通过PCR检测到的基因组存在与感染性病毒颗粒之间的相关性。这突出了PCR作为感染性病毒存在的可靠指标的潜力。然而,这种感染性测定证明,即使PCR结果检测不到,仍可能有数量减少的病毒保持活力并能够感染培养中的易感细胞。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c734/11643883/afb447ca62a6/molecules-29-05601-g001.jpg

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