Almukhova E A, Vorob'eva L I
Mikrobiologiia. 1985 Mar-Apr;54(2):191-6.
Biotin transport was studied in the yeast Trichosporon cutaneum and was shown to be the active transport with Km = 1.5 X 10(-7) M. The process depended on the pH and temperature whose optimal values were pH 6.8-7.2 and 35-40 degrees C. T. cutaneum biotin permease was highly specific for biotin and was inhibited by biotin analogs. Biotin uptake by the cells was inhibited by glucose. Excess biotin in the growth medium caused partial repression of its transport system in the yeast. Synthesis of biotin permease by T. cutaneum depended on the nature of a catabolite present in the growth medium. The rate of biotin transport was highest in the cells grown in the presence of ramnose or arabinose and lowest in the cells utilizing galactose, lactose or xylose.
对皮状丝孢酵母中的生物素转运进行了研究，结果表明其为主动转运，米氏常数（Km）为1.5×10⁻⁷ M。该过程依赖于pH值和温度，其最佳值分别为pH 6.8 - 7.2和35 - 40℃。皮状丝孢酵母生物素通透酶对生物素具有高度特异性，并受到生物素类似物的抑制。细胞对生物素的摄取受到葡萄糖的抑制。生长培养基中过量的生物素会导致酵母中其转运系统的部分抑制。皮状丝孢酵母生物素通透酶的合成取决于生长培养基中存在的分解代谢物的性质。在以鼠李糖或阿拉伯糖为碳源生长的细胞中，生物素转运速率最高，而在利用半乳糖、乳糖或木糖的细胞中最低。
