Catalases () are present in almost all living organisms and play important roles in plant development and response to various stresses. However, there is relatively little information on genes in wheat and related species. A few studies on family genes in wheat have been reported. In this study, ten proteins () were identified in wheat and classified into three groups based on their phylogenetic features and sequence analysis. The analysis of the structure and motif composition of the proteins suggested that a segmental duplication event occurred in the gene family. Collinearity relationship analysis among different species showed that there were three orthologous genes in rice and in maize. By analyzing the -elements in the promoter regions, we speculated that genes expression might be regulated by light, oxygen deficit, methyl jasmonate and abscisic acid, and by transcription factors such as MYB. A Gene Ontology (GO)-based analysis showed that proteins may be related to the response to various stresses, are cytoplasm localized, and may function as antioxidant enzymes. RT-qPCR and transcriptome data analyses exhibited distinct expression patterns of genes in different tissues and in response to various treatments. In this study, a comprehensive analysis of wheat genes was performed, enriching our knowledge of genes and providing a foundation for further functional analyses of this gene family in wheat.