Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing 100083, China.
Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, China.
Int J Mol Sci. 2024 Aug 31;25(17):9504. doi: 10.3390/ijms25179504.
The Russian olive (), which functions as a "dead-end trap tree" for the Asian long-horned beetle () in mixed plantations, can successfully attract Asian long-horned beetles for oviposition and subsequently kill the eggs by gum. This study aimed to investigate gum secretion differences by comparing molecular and metabolic features across three conditions-an oviposition scar, a mechanical scar, and a healthy branch-using high-performance liquid chromatography and high-throughput RNA sequencing methods. Our findings indicated that the gum mass secreted by an oviposition scar was 1.65 times greater than that secreted by a mechanical scar. Significant differences in gene expression and metabolism were observed among the three comparison groups. A Kyoto Encyclopedia of Genes and Genomes annotation and enrichment analysis showed that an oviposition scar significantly affected starch and sucrose metabolism, leading to the discovery of 52 differentially expressed genes and 7 differentially accumulated metabolites. A network interaction analysis of differentially expressed metabolites and genes showed that , , and regulate sucrose, uridine diphosphate glucose, α-D-glucose-1P, and D-glucose-6P. Although the polysaccharide content in the OSs was 2.22 times higher than that in the MSs, the sucrose content was lower. The results indicated that the Asian long-horned beetle causes Russian olive sucrose degradation and D-glucose-6P formation. Therefore, we hypothesized that damage caused by the Asian long-horned beetle could enhance tree gum secretions through hydrolyzed sucrose and stimulate the Russian olive's specific immune response. Our study focused on the first pair of a dead-end trap tree and an invasive borer pest in forestry, potentially offering valuable insights into the ecological self-regulation of Asian long-horned beetle outbreaks.
沙枣（）作为一种“死胡同陷阱树”，在混交林中专一为亚洲长角天牛（）提供产卵场所，能够成功吸引亚洲长角天牛产卵，并通过树胶杀死卵。本研究旨在通过比较三种情况（产卵痕、机械痕和健康枝条）的分子和代谢特征，使用高效液相色谱和高通量 RNA 测序方法，研究胶分泌差异。我们的研究结果表明，产卵痕分泌的胶质量比机械痕多 1.65 倍。在三个比较组之间观察到基因表达和代谢的显著差异。京都基因与基因组百科全书注释和富集分析表明，产卵痕显著影响淀粉和蔗糖代谢，发现 52 个差异表达基因和 7 个差异积累代谢物。差异表达代谢物和基因的网络互作分析表明， 、 、 调节蔗糖、尿苷二磷酸葡萄糖、α-D-葡萄糖-1P 和 D-葡萄糖-6P。尽管 OSs 中的多糖含量比 MSs 高 2.22 倍，但蔗糖含量较低。结果表明，亚洲长角天牛导致沙枣蔗糖降解和 D-葡萄糖-6P 形成。因此，我们假设亚洲长角天牛的损伤可以通过水解蔗糖来增强树木胶的分泌，并刺激沙枣的特定免疫反应。我们的研究集中在林业中第一对死胡同陷阱树和入侵蛀干害虫上，这可能为亚洲长角天牛爆发的生态自我调节提供有价值的见解。
