Siwak Mateusz, Piotrzkowska Danuta, Skrzypek Maciej, Majsterek Ireneusz
Department of Clinical Chemistry and Biochemistry, Medical University of Lodz, 92-215 Lodz, Poland.
Int J Mol Sci. 2024 Nov 28;25(23):12791. doi: 10.3390/ijms252312791.
Peripheral nerve regeneration remains a major challenge in neuroscience, despite advancements in understanding its mechanisms. Current treatments, including nerve transplantation and drug therapies, face limitations such as invasiveness and incomplete recovery of nerve function. Physical therapies, like pulsed electromagnetic fields (PEMF) and low-intensity ultrasound (LIPUS), are gaining attention for their potential to enhance regeneration. This study analyzes the effects of PEMF and LIPUS on gene expression in human primary Schwann cells, which are crucial for nerve myelination and repair. Key genes involved in neurotrophin signaling (NGF, BDNF), inflammation (IL-1β, IL-6, IL-10, TNF-α, TGF-β), and regeneration (CRYAB, CSPG, Ki67) were assessed. The results of this study reveal that combined PEMF and LIPUS therapies promote Schwann cell proliferation, reduce inflammation, and improve the regenerative environment, offering potential for optimizing these therapies for clinical use in regenerative medicine.
尽管在理解其机制方面取得了进展，但周围神经再生仍然是神经科学中的一项重大挑战。目前的治疗方法，包括神经移植和药物治疗，面临着诸如侵入性和神经功能恢复不完全等局限性。物理治疗，如脉冲电磁场（PEMF）和低强度超声（LIPUS），因其增强再生的潜力而受到关注。本研究分析了PEMF和LIPUS对人原代雪旺细胞基因表达的影响，雪旺细胞对神经髓鞘形成和修复至关重要。评估了参与神经营养因子信号传导（NGF、BDNF）、炎症（IL-1β、IL-6、IL-10、TNF-α、TGF-β）和再生（CRYAB、CSPG、Ki67）的关键基因。本研究结果表明，联合PEMF和LIPUS疗法可促进雪旺细胞增殖、减轻炎症并改善再生环境，为优化这些疗法在再生医学中的临床应用提供了潜力。
