Xu Xiuyue, Su Hong, Sun Shuwei, Sun Jing, Zhang Xiang, Yu Jiajie
School of Agriculture, Liaodong University, Dandong 118003, China.
Forestry College, Northeast Forestry University, Harbin 150040, China.
Int J Mol Sci. 2024 Nov 28;25(23):12832. doi: 10.3390/ijms252312832.
Nuclear Factor Y A (NF-YA) transcription factors are widely involved in multiple plant biological processes, such as embryogenesis, abscisic acid signaling, and abiotic stress response. This study presents a comprehensive genome-wide identification and expression profiling of NF-YA transcription factors in blueberry (), an important economic crop with good adaptability, under abiotic stress conditions. Given the economic significance and health benefits of blueberries, understanding their responses to environmental stresses, such as salt, drought, and temperature extremes, is crucial. A total of 24 NF-YA transcription factors were identified through bioinformatics analyses, including sequence alignment, phylogenetic tree construction, and conserved motif analysis. The expression patterns of these genes were evaluated in various tissues (roots, stems, and leaves) and under different stress treatments (abscisic acid, salt, and cold) using quantitative real-time PCR (qRT-PCR). The results indicated that most genes exhibited higher expression levels in stems and leaves compared to roots. Most s were responsive to the stress treatment. Furthermore, -acting element analysis revealed that the promoters of s were enriched with elements responsive to abiotic stress, suggesting their pivotal role in stress adaptation. This research unveils the expressional responses of NF-YA transcription factors in blueberry upon abiotic stresses and lays the groundwork for future studies on improving crop adaptation.
核因子Y A（NF-YA）转录因子广泛参与多种植物生物学过程，如胚胎发生、脱落酸信号传导和非生物胁迫响应。本研究对蓝莓（一种适应性良好的重要经济作物）在非生物胁迫条件下的NF-YA转录因子进行了全基因组范围的综合鉴定和表达谱分析。鉴于蓝莓的经济意义和健康益处，了解它们对盐、干旱和极端温度等环境胁迫的反应至关重要。通过生物信息学分析，包括序列比对、系统发育树构建和保守基序分析，共鉴定出24个NF-YA转录因子。使用定量实时PCR（qRT-PCR）评估了这些基因在不同组织（根、茎和叶）以及不同胁迫处理（脱落酸、盐和冷）下的表达模式。结果表明，与根相比，大多数基因在茎和叶中表现出更高的表达水平。大多数基因对胁迫处理有响应。此外，顺式作用元件分析表明，这些基因的启动子富含对非生物胁迫响应的元件，表明它们在胁迫适应中起关键作用。本研究揭示了蓝莓中NF-YA转录因子在非生物胁迫下的表达响应，并为未来改善作物适应性的研究奠定了基础。
