Liu Yi-Fang, Wu Yi-Chen, Yang Yu, Lo Hui-Chen
Department of Nutritional Science, Fu Jen Catholic University, New Taipei City 242062, Taiwan.
Department of Food and Nutrition, Tri-Service General Hospital, Taipei 114202, Taiwan.
Int J Mol Sci. 2024 Dec 3;25(23):12977. doi: 10.3390/ijms252312977.
A plant-based diet is considered a promising approach for managing hyperuricemia (HUA). This study examined the effects of soy protein and plant-based oils on HUA-induced inflammation and immune dysfunction. Male Wistar rats, induced with HUA using oxonic acid and uric acid (UA), were fed casein or soy protein with palm or safflower oil (2 × 2 factorial design) for 8 weeks. HUA rats had lower serum albumin and T cell percentages in peripheral blood leukocytes (PBLs) and splenocytes, along with increased leukocyte counts and spleen weights, compared to healthy rats ( < 0.05). Soy protein improved HUA-induced reductions in albumin, while safflower-seed oil ameliorated reductions in albumin, plasma interleukin (IL)-4, and T-suppressor splenocytes, and mitigated elevated serum UA, plasma IL-6, and B leukocytes (two-way ANOVA, < 0.05). In PBL, soy protein alleviated HUA-induced decreases in TNF-α, casein and palm oil increased IL-6, and casein further reduced IFN-γ production. Under Con A stimulation, casein and safflower-seed oil alleviated decreases in IL-6 and IL-10, respectively, while under LPS stimulation, casein further increased TNF-α production. In splenocytes, soy protein and safflower-seed oil reduced HUA-induced increases in TNF-α and increased IL-10, and safflower-seed oil increased IL-6 production. Under Con A stimulation, soy protein and safflower-seed oil reduced TNF-α and increased IL-10 production in splenocytes. The findings suggest that soy protein and safflower-seed oil may counteract HUA-related inflammation, alleviate monocyte activation, and enhance Th2 immune response in HUA. A plant-based diet rich in soy protein and safflower-seed oil may help manage HUA and associated inflammation and immune dysfunction.
以植物为基础的饮食被认为是管理高尿酸血症（HUA）的一种有前景的方法。本研究考察了大豆蛋白和植物油对HUA诱导的炎症和免疫功能障碍的影响。使用氧嗪酸和尿酸（UA）诱导HUA的雄性Wistar大鼠，分别喂食酪蛋白或大豆蛋白以及棕榈油或红花油（2×2析因设计），持续8周。与健康大鼠相比，HUA大鼠外周血白细胞（PBL）和脾细胞中的血清白蛋白和T细胞百分比更低，白细胞计数和脾脏重量增加（P<0.05）。大豆蛋白改善了HUA诱导的白蛋白降低，而红花籽油改善了白蛋白、血浆白细胞介素（IL）-4和抑制性T脾细胞的降低，并减轻了血清UA升高、血浆IL-6和B淋巴细胞升高（双向方差分析，P<0.05）。在PBL中，大豆蛋白减轻了HUA诱导的肿瘤坏死因子-α（TNF-α）降低，酪蛋白和棕榈油增加了IL-6，酪蛋白进一步降低了干扰素-γ（IFN-γ）的产生。在刀豆蛋白A（Con A）刺激下，酪蛋白和红花籽油分别减轻了IL-6和IL-10的降低，而在脂多糖（LPS）刺激下，酪蛋白进一步增加了TNF-α的产生。在脾细胞中，大豆蛋白和红花籽油降低了HUA诱导的TNF-α增加并增加了IL-10，红花籽油增加了IL-6的产生。在Con A刺激下，大豆蛋白和红花籽油降低了脾细胞中TNF-α并增加了IL-10的产生。这些发现表明，大豆蛋白和红花籽油可能抵消HUA相关的炎症，减轻单核细胞活化，并增强HUA中的Th2免疫反应。富含大豆蛋白和红花籽油的植物性饮食可能有助于管理HUA以及相关的炎症和免疫功能障碍。
