Zhu Xi, Duan Huimin, Zhang Guodong, Jin Hui, Xu Chao, Chen Shu, Zhou Chuanmeng, Chen Zhuo, Tang Jinghua, Zhang Yu
Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture and Rural Affairs of China/Key Laboratory of Hainan Province for Postharvest Physiology and Technology of Tropical Horticultural Products, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, China.
National Key Laboratory for Tropical Crop Breeding, Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya, China.
Front Plant Sci. 2023 Jun 20;14:1218962. doi: 10.3389/fpls.2023.1218962. eCollection 2023.
Mitogen-activated protein kinases (MAPKs) have been reported to respond to various stimuli including heat stress. This research aimed to investigate whether is implicated in the transduction of the heat stress signal to adapt heat stress as a thermos-tolerant gene.
Potato plants were cultivated under mild (30°C) and acute (35°C) heat stress conditions to analyze mRNA expression of and physiological indicators. was up-regulated and down-regulated by transfection. Subcellular localization of StMAPK1 protein was observed by fluorescence microscope. The transgenic potato plants were assayed for physiological indexes, photosynthesis, cellular membrane integrity, and heat stress response gene expression.
Heat stress altered the expression prolife of . overexpression changed the physiological characteristics and phenotypes of potato plants under heat stresses. mediates photosynthesis and maintains membrane integrity of potato plants in response to heat stress. Stress response genes (, , , and ) in potato plants were altered by dysregulation. mRNA expression of heat stress genes (, , , and ) was affected by .
overexpression increases the heat-tolerant capacity of potato plants at the morphological, physiological, molecular, and genetic levels.
有报道称丝裂原活化蛋白激酶（MAPKs）可对包括热应激在内的多种刺激作出反应。本研究旨在探究[具体基因名称未给出]是否参与热应激信号的转导，作为一种耐热基因来适应热应激。
将马铃薯植株种植在轻度（30°C）和急性（35°C）热应激条件下，以分析[具体基因名称未给出]的mRNA表达和生理指标。通过转染使[具体基因名称未给出]上调和下调。利用荧光显微镜观察StMAPK1蛋白的亚细胞定位。对转基因马铃薯植株的生理指标、光合作用、细胞膜完整性和热应激反应基因表达进行测定。
热应激改变了[具体基因名称未给出]的表达模式。[具体基因名称未给出]过表达改变了热应激下马铃薯植株的生理特征和表型。[具体基因名称未给出]介导光合作用并维持热应激下马铃薯植株的膜完整性。马铃薯植株中的应激反应基因（[具体基因名称未给出]）因[具体基因名称未给出]失调而发生改变。热应激基因（[具体基因名称未给出]）的mRNA表达受[具体基因名称未给出]影响。
[具体基因名称未给出]过表达在形态、生理、分子和遗传水平上提高了马铃薯植株的耐热能力。
