Lee Eun Hye, Kim Yu Jin, Jung Il Seon, Kim Dae Keun, Lee Jae Ho
Department of Biomedical Science, College of Life Science, CHA University, Pocheon 11160, Gyeonggi-do, Republic of Korea.
CHA Fertility Center, Seoul Station, Hangang-daero, Jung-gu, Seoul 04637, Republic of Korea.
Int J Mol Sci. 2024 Dec 9;25(23):13220. doi: 10.3390/ijms252313220.
Idiopathic male infertility is characterized by increased mortality or reduced motility and vitality of sperm. There are several reports on probiotics in the male reproductive tract, but the effects of these probiotics on sperm motility remain to be elucidated. In this study, we investigated the impact and mechanism of probiotics on the vitality and motility of mouse sperm. We collected mature sperm from the caudal vas deferens of mice and prepared three probiotics donated by HEM Pharma Inc.: , , and . We analyzed the vitality and motility of sperm according to the concentration and duration of probiotic treatment. The probiotics increased the motility and vitality of sperm. Specifically, they enhanced sperm motility by 30-40% compared with untreated sperms. The probiotics enhanced mitochondrial activity in sperm through specific factors like AMPK and SIRT1. All three probiotics enhanced the activities of mitochondrial function-related proteins in sperm. In conclusion, we found that the probiotics improved the vitality and motility of mouse sperm and increased mitochondrial function in mature sperm. These findings suggest that probiotics can be utilized to enhance sperm motility and treat male infertility.
特发性男性不育的特征是死亡率增加或精子活力和生存力降低。关于男性生殖道中的益生菌已有多项报道，但这些益生菌对精子活力的影响仍有待阐明。在本研究中，我们调查了益生菌对小鼠精子活力和运动能力的影响及其机制。我们从小鼠的输精管尾部收集成熟精子，并制备了由HEM制药公司捐赠的三种益生菌： 、 和 。我们根据益生菌处理的浓度和持续时间分析了精子的活力和运动能力。益生菌提高了精子的活力和运动能力。具体而言，与未处理的精子相比，它们使精子活力提高了30-40%。益生菌通过AMPK和SIRT1等特定因子增强了精子中的线粒体活性。所有三种益生菌都增强了精子中线粒体功能相关蛋白的活性。总之，我们发现益生菌改善了小鼠精子的活力和运动能力，并增加了成熟精子的线粒体功能。这些发现表明，益生菌可用于提高精子活力和治疗男性不育症。
