School of Industrial Technology, Universiti Sains Malaysia, Penang, Malaysia.
USM-RIKEN International Centre for Ageing Science (URICAS), Universiti Sains Malaysia, Penang, Malaysia.
J Appl Microbiol. 2021 Apr;130(4):1307-1322. doi: 10.1111/jam.14776. Epub 2020 Sep 20.
The aim of this study was to evaluate the molecular mechanisms of Lactobacillus strains in improving ageing of the musculoskeletal system.
The anti-ageing mechanism of three probiotics strains Lactobacillus fermentum DR9, Lactobacillus paracasei OFS 0291 and L. helveticus OFS 1515 were evaluated on gastrocnemius muscle and tibia of d-galactose-induced ageing rats. Upon senescence induction, aged rats demonstrated reduced antioxidative genes CAT and SOD expression in both bone and muscle compared to the young rats (P < 0·05). Strain L. fermentum DR9 demonstrated improved expression of SOD in bone and muscle compared to the aged rats (P < 0·05). In the evaluation of myogenesis-related genes, L. paracasei OFS 0291 and L. fermentum DR9 increased the mRNA expression of IGF-1; L. helveticus OFS 1515 and L. fermentum DR9 reduced the expression of MyoD, in contrast to the aged controls (P < 0·05). Protective effects of L. fermentum DR9 on ageing muscle were believed to be contributed by increased AMPK-α2 expression. Among the osteoclastogenesis genes studied, TNF-α expression was highly elevated in tibia of aged rats, while all three probiotics strains ameliorated the expression. Lactobacillus fermentum DR9 also reduced the expression of IL-6 and TRAP in tibia when compared to the aged rats (P < 0·05). All probiotics treatment resulted in declined proinflammatory cytokines IL-1β in muscle and bone.
Lactobacillus fermentum DR9 appeared to be the strongest strain in modulation of musculoskeletal health during ageing.
The study demonstrated the protective effects of the bacteria on muscle and bone through antioxidative and anti-inflammatory actions. Therefore, L. fermentum DR9 may serve as a promising targeted anti-ageing therapy.
本研究旨在评估乳酸菌菌株改善肌肉骨骼系统衰老的分子机制。
在半乳糖诱导衰老大鼠的比目鱼肌和胫骨上评估了三种益生菌菌株干酪乳杆菌 DR9、副干酪乳杆菌 OFS 0291 和瑞士乳杆菌 OFS 1515 的抗衰老机制。衰老诱导后，与年轻大鼠相比，衰老大鼠的骨和肌肉中的抗氧化基因 CAT 和 SOD 表达降低（P<0.05）。与衰老大鼠相比，干酪乳杆菌 DR9 显示出骨和肌肉中 SOD 表达的改善（P<0.05）。在肌生成相关基因的评估中，副干酪乳杆菌 OFS 0291 和干酪乳杆菌 DR9 增加了 IGF-1 的 mRNA 表达；与衰老对照组相比，瑞士乳杆菌 OFS 1515 和干酪乳杆菌 DR9 降低了 MyoD 的表达（P<0.05）。干酪乳杆菌 DR9 对衰老肌肉的保护作用可能归因于 AMPK-α2 表达的增加。在所研究的破骨细胞生成基因中，衰老大鼠胫骨中 TNF-α 的表达高度升高，而所有三种益生菌菌株均改善了其表达。与衰老大鼠相比，干酪乳杆菌 DR9 还降低了胫骨中 IL-6 和 TRAP 的表达（P<0.05）。所有益生菌处理均导致肌肉和骨骼中促炎细胞因子 IL-1β 的表达下降。
干酪乳杆菌 DR9 似乎是调节衰老过程中肌肉骨骼健康的最强菌株。
该研究通过抗氧化和抗炎作用证明了细菌对肌肉和骨骼的保护作用。因此，干酪乳杆菌 DR9 可能是一种有前途的靶向抗衰老治疗方法。
