Gaviria-Cantin Tania, Vargas Andrés Felipe, Mouali Youssef El, Jiménez Carlos Jonay, Cimdins-Ahne Annika, Madrid Cristina, Römling Ute, Balsalobre Carlos
Department of Genetics, Microbiology and Statistics, School of Biology, University of Barcelona, Av. Diagonal 643, 08028 Barcelona, Spain.
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, 17177 Stockholm, Sweden.
Microorganisms. 2022 Sep 27;10(10):1921. doi: 10.3390/microorganisms10101921.
Rdar biofilm formation of and is a common ancient multicellular behavior relevant in cell-cell and inter-organism interactions equally, as in interaction with biotic and abiotic surfaces. With the expression of the characteristic extracellular matrix components amyloid curli fimbriae and the exopolysaccharide cellulose, the central hub for the delicate regulation of rdar morphotype expression is the orphan transcriptional regulator CsgD. Gre factors are ubiquitously interacting with RNA polymerase to selectively overcome transcriptional pausing. In this work, we found that GreA/GreB are required for expression of the operon and consequently the rdar morphotype. The ability of the Gre factors to suppress transcriptional pausing and the 147 bp 5'-UTR of are required for the stimulatory effect of the Gre factors on expression. These novel mechanism(s) of regulation for the operon might be relevant under specific stress conditions.
Rdar生物膜的形成是一种常见的古老多细胞行为，同样与细胞间和生物体间的相互作用相关，就像与生物和非生物表面的相互作用一样。随着特征性细胞外基质成分淀粉样卷曲菌毛和胞外多糖纤维素的表达，孤儿转录调节因子CsgD是rdar形态型表达精细调控的中心枢纽。Gre因子与RNA聚合酶普遍相互作用，以选择性地克服转录暂停。在这项工作中，我们发现GreA/GreB是操纵子表达以及rdar形态型所必需的。Gre因子抑制转录暂停的能力和操纵子147 bp的5'-UTR对于Gre因子对操纵子表达的刺激作用是必需的。操纵子的这些新型调控机制可能在特定应激条件下具有相关性。
