Keisham Sunada, Tateno Hiroaki
Cellular and Molecular Biotechnology Research Institute, Multicellular System Regulation Research Group, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
Ph.D. Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba, Tsukuba, Japan.
BBA Adv. 2024 Nov 26;6:100125. doi: 10.1016/j.bbadva.2024.100125. eCollection 2024.
Glycans are present on virtually all cellular surfaces and are important regulators of multicellular communications. Advances in single-cell omics technologies have revolutionized life science research by elucidating cellular heterogeneity through integrated multimodal analyses, providing a comprehensive view of cellular functions. However, dissecting the heterogeneity of glycans at the single-cell level has been challenging due to their structural complexity and unamplifiable nature. Recently, we developed a novel technology called single-cell glycan and RNA sequencing (scGR-seq), which converts glycan information into genetic information using DNA-barcoded lectins, amplifies it by PCR, and simultaneously measures the glycome and transcriptome in thousands of single cells on a next-generation sequencer. In this mini-review, we review the recent advances in single-cell glycomics, focusing on our scGR-seq technology.
聚糖几乎存在于所有细胞表面，是多细胞通讯的重要调节因子。单细胞组学技术的进步通过整合多模态分析阐明细胞异质性，为细胞功能提供了全面的视角，从而彻底改变了生命科学研究。然而，由于聚糖结构复杂且不可扩增，在单细胞水平剖析聚糖的异质性一直具有挑战性。最近，我们开发了一种名为单细胞聚糖和RNA测序（scGR-seq）的新技术，该技术使用DNA条形码凝集素将聚糖信息转化为遗传信息，通过PCR进行扩增，并在下一代测序仪上同时测量数千个单细胞中的糖组和转录组。在本综述中，我们回顾了单细胞糖组学的最新进展，重点介绍了我们的scGR-seq技术。
