Nafchi Hanieh Ghasemian, Azizi Yaser, Halvaei Iman
Department of Physiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Reprod Sci. 2024 Jun;31(6):1586-1592. doi: 10.1007/s43032-024-01505-y. Epub 2024 Mar 6.
The cryopreservation procedure decreases sperm quality, causing certain changes at structural and molecular levels affecting fertilizing ability. We aimed to investigate the impacts of human adipose-derived mesenchymal stem cells (HAd-MSCs) conditioned medium (CM) on the protection of human sperm from cryoinjury. Thirty normal semen specimens were evaluated in this study. Each specimen was separated into six groups and enhanced with varying concentrations of human Ad-MSCs-CM (0, 10, 30, 50, 70, and 100%). Sperm motility, viability, morphology, apoptosis, mitochondrial potential, and lipid peroxidation, and DNA fragmentation were evaluated before freezing and after thawing. The results showed that the total motility was preserved in 10% human Ad-MSCs-CM group. Also, DNA fragmentation was significantly lower in 10% compared to 0% human Ad-MSCs-CM (63.62 ± 17.72% vs.76.46 ± 4.87%, respectively, P < 0.004). Human Ad-MSCs-CM in groups of 10, 30, 50, and 70% reduced lipid peroxidation. The normal sperm morphology rate, mitochondrial membrane potential, and apoptosis showed no significant differences across various groups. It seems that human Ad-MSCs-CM can protect the sperm parameters during the cryopreservation by decreasing cryoinjury.
冷冻保存程序会降低精子质量，在结构和分子水平上引起某些变化，从而影响受精能力。我们旨在研究人脂肪间充质干细胞（HAd-MSCs）条件培养基（CM）对保护人类精子免受冷冻损伤的影响。本研究评估了30份正常精液标本。每份标本分为六组，并用不同浓度的人Ad-MSCs-CM（0、10、30、50、70和100%）进行强化处理。在冷冻前和解冻后评估精子活力、存活率、形态、凋亡、线粒体电位、脂质过氧化和DNA片段化情况。结果显示，10%人Ad-MSCs-CM组的总活力得以保留。此外，与0%人Ad-MSCs-CM相比，10%组的DNA片段化显著更低（分别为63.62±17.72%和76.46±4.87%，P<0.004）。10%、30%、50%和70%组的人Ad-MSCs-CM降低了脂质过氧化。正常精子形态率、线粒体膜电位和凋亡在各组之间无显著差异。人Ad-MSCs-CM似乎可以通过减少冷冻损伤来保护冷冻保存过程中的精子参数。
