Cheng Shu-Chen, Liou Chian-Jiun, Wu Shu-Ju, Lin Chwan-Fwu, Huang Tse-Hung, Huang Wen-Chung
Graduate Institute of Health Industry Technology, Research Center for Chinese Herbal Medicine, Chang Gung University of Science and Technology, No.261, Wenhua 1st Rd., Taoyuan City 33303, Taiwan; Department of Traditional Chinese Medicine, Chang Gung Memorial Hospital, Taoyuan 33303, Taiwan.
Department of Nursing, Division of Basic Medical Sciences, Research Center for Chinese Herbal Medicine, Chang Gung University of Science and Technology, No.261, Wenhua 1st Rd., Taoyuan City 33303, Taiwan; Division of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital, Linkou, Taoyuan City 33303, Taiwan.
Int Immunopharmacol. 2025 Jan 27;146:113867. doi: 10.1016/j.intimp.2024.113867. Epub 2024 Dec 16.
Neochlorogenic acid is a natural compound isolated from various fruits and vegetables that has anti-inflammation and anti-oxidative effects in macrophages. Inflammatory immune cells and tracheal epithelial cells can stimulate airway hyperresponsiveness, inflammation, and reactive oxygen species. In this study, we investigated the effect of neochlorogenic acid in ameliorating inflammatory and oxidative responses in asthmatic mice. We used an ovalbumin (OVA)-induced mouse model, treating mice with neochlorogenic acid by intraperitoneal injection. We also treated inflammatory human tracheal epithelial (BEAS-2B) cells with neochlorogenic acid to evaluate inflammatory cytokine levels and oxidative responses. The results demonstrate that neochlorogenic acid attenuated airway hyperresponsiveness, eosinophil infiltration, and goblet cell hyperplasia in the lungs of asthmatic mice. Neochlorogenic acid also reduced type 2 cytokine expression in bronchoalveolar lavage fluid and improved oxidative stress in the lung. Neochlorogenic acid effectively blocked monocyte attachment to adherent BEAS-2B cells, and reduced pro-inflammatory cytokine and reactive oxygen species production in inflammatory BEAS-2B cells. These findings suggest that neochlorogenic acid is a potential immunomodulator that can ameliorate airway hyperresponsiveness and airway inflammation in asthmatic mice.
新绿原酸是一种从多种水果和蔬菜中分离出的天然化合物，在巨噬细胞中具有抗炎和抗氧化作用。炎性免疫细胞和气管上皮细胞可刺激气道高反应性、炎症和活性氧的产生。在本研究中，我们调查了新绿原酸对改善哮喘小鼠炎症和氧化反应的作用。我们使用卵清蛋白（OVA）诱导的小鼠模型，通过腹腔注射用新绿原酸处理小鼠。我们还用新绿原酸处理炎性人气管上皮（BEAS-2B）细胞，以评估炎性细胞因子水平和氧化反应。结果表明，新绿原酸减轻了哮喘小鼠肺部的气道高反应性、嗜酸性粒细胞浸润和杯状细胞增生。新绿原酸还降低了支气管肺泡灌洗液中2型细胞因子的表达，并改善了肺部的氧化应激。新绿原酸有效阻断单核细胞与贴壁BEAS-2B细胞的附着，并减少炎性BEAS-2B细胞中促炎细胞因子和活性氧的产生。这些发现表明，新绿原酸是一种潜在的免疫调节剂，可改善哮喘小鼠的气道高反应性和气道炎症。
