Bandeira Tereza de Jesus Pinheiro Gomes, Oliveira Ana Leticia Sousa de, Martins Luis Fernando Pontes, Matos Rodrigo Moreira, Santos Sheryda Raynna Nobre Guedes Dos, Lopes Mateus Cedro, Sobreira Raimundo Tadeu Pires, Rocha Hermano Alexandre Lima
Unichristus: Centro Universitario Christus, Fortaleza, Brazil.
Braz J Microbiol. 2025 Mar;56(1):537-543. doi: 10.1007/s42770-024-01581-x. Epub 2024 Dec 17.
Respiratory tract infection (RTI) is the most important cause of visiting both public and private medical care services. During the decade preceding Covid-19, in developing countries, there was a gap in the epidemiology of respiratory viral infections, probably due to the difficulty of detecting viral agents in clinical microbiology laboratories routine, at that time. In this context, the aim of this study was to describe the occurrence of virus, demographic factors associated and the likelihood of coinfections detection by multiplex PCR methods. The target also was to determine the involvement of respiratory viruses other than SARS-CoV-2 as agents of upper respiratory tract infections (URTIs) using rapid respiratory panels by multiplex real time PCR with flow chip methodology. Samples of SARSs patients, 530 in total, were tested between 2022 and 2023, obtained from nasopharyngeal swabs, nasopharyngeal exudates or nasopharyngeal aspirates. Of 530 tests, 30% was human rhinovirus, the most prevalent, followed by respiratory syncytial virus B (21.5%) and human adenovirus (17.4%). Among the total of positive samples, 83.8% belonged to patients aged between 0 and 10 years, 62.6% of whom were aged between 1 and 10 years. Multiple virus detection was found in 50.4% of the samples tested, 93.1% of which were from patients under the age of 18. Viral co-infection was evident in the finding and was higher (88.7%) in patients under the age of 18 yeas.
呼吸道感染（RTI）是人们前往公共和私人医疗服务机构就诊的最重要原因。在新冠疫情之前的十年里，在发展中国家，呼吸道病毒感染的流行病学存在差距，这可能是由于当时临床微生物实验室常规检测病毒病原体存在困难。在此背景下，本研究的目的是描述病毒的发生情况、相关的人口统计学因素以及通过多重PCR方法检测合并感染的可能性。目标还包括使用具有流式芯片技术的多重实时PCR快速呼吸道检测板，确定除新冠病毒外的呼吸道病毒作为上呼吸道感染（URTI）病原体的情况。2022年至2023年期间，共对530例严重急性呼吸综合征（SARS）患者的样本进行了检测，样本取自鼻咽拭子、鼻咽分泌物或鼻咽抽吸物。在530次检测中，30%为人类 rhinovirus，最为常见，其次是呼吸道合胞病毒B（21.5%）和人类腺病毒（17.4%）。在所有阳性样本中，83.8%属于0至10岁的患者，其中62.6%年龄在1至10岁之间。在50.4%的检测样本中发现了多种病毒检测结果，其中93.1%来自18岁以下的患者。在检测结果中明显存在病毒合并感染，18岁以下患者中的合并感染率更高（88.7%）。
