Skin-associated shares cobamides.

UNLABELLED

The underlying interactions that occur to maintain skin microbiome composition, function, and overall skin health are largely unknown. Often, these types of interactions are mediated by microbial metabolites. Cobamides, the vitamin B family of cofactors, are essential for metabolism in many bacteria but are only synthesized by a fraction of prokaryotes, including certain skin-associated species. Therefore, we hypothesize that cobamide sharing mediates skin community dynamics. Preliminary work predicts that several skin-associated species encode cobamide biosynthesis and that their abundance is associated with skin microbiome diversity. Here, we show that commensal produces cobamides and that this synthesis can be tuned by cobalt limitation. To demonstrate cobamide sharing by , we employed a co-culture assay using an cobamide auxotroph and showed that produces sufficient cobamides to support growth, both in liquid co-culture and when separated spatially on solid medium. We also generated a non-cobamide-producing strain (cob) using UV mutagenesis that contains mutated cobamide biosynthesis genes (precorrin-6X reductase) and (corrinoid adenosyltransferase) and confirm that disruption of cobamide biosynthesis abolishes the support of growth through cobamide sharing. Our study provides a unique model to study metabolite sharing by microorganisms, which will be critical for understanding the fundamental interactions that occur within complex microbiomes and for developing approaches to target the human microbiota for health advances.

IMPORTANCE

The human skin serves as a crucial barrier for the body and hosts a diverse community of microbes known as the skin microbiome. The interactions that occur to maintain a healthy skin microbiome are largely unknown but are thought to be driven in part, by nutrient sharing between species in close association. Here we show that the skin-associated bacteria produces and shares cobalamin, a cofactor essential for survival in organisms across all domains of life. This study provides a unique model to study metabolite sharing by skin microorganisms, which will be critical for understanding the fundamental interactions that occur within the skin microbiome and for developing therapeutic approaches aiming to engineer and manipulate the skin microbiota.